Effect Of Gastrodin Intervention On Methamphetamine Dependence Rats Through The Expression Of BDNF And NGF In Related Brain Regions~*

Objective:To establish the methamphetamine dependent rat model, using gastrodin to intervene the rats model. To detect the expression of brain-derived neurotrophic factor(BDNF) and nerve growth factor(NGF) in six cerebral regions, which were frontal cortex, striatum, nucleus accumbens, hippocampus, substantia nigra and ventral tegmental. Investigating whether gastrodin has the protective or therapeutic effect on methamphetamine dependence rat. Methods:Ninety SD rats were divided into 9 groups, which were control group, one week MA-dependent group, two weeks MA-dependent group, four weeks MA-dependent group, eight weeks MA-dependent group, one week MA+gastrodin group, two weeks MA+gastrodin group, four weeks MA+gastrodin group and eight weeks MA+gastrodin group(n=10). Intraperitoneal injection of methamphetamine to establish rat model of methamphetamine dependence, conditioned place preference experiment (CPP) and stereotyped behavior were performed to confirm methamphetamine dependence. The methods of western blotting and real-time quantitative PCR were used to detect the expression of BDNF and NGF in six cerebral regions. Conclusion:1. The methamphetamine dependent rat model could be established successfully through conditioned place preference experiment and the stereotyped behavior scores.2. Different brain regions of MA dependent rats (which were nucleus accumbens, striatum, hippocampus, frontal cortex, substantia nigra, and ventral tegmental area), the expressions of BDNF and NGF had changed significantly. The results showed there was correlation between BDNF and NGF contents and the neurotoxicity induced by MA in rat brain. 3.The expression of BDNF and NGF in six brain regions showed a rising tendency after the intervention of the gastrodin. It indicated that gastrodin had a therapeutic effect on methamphetamine induced neurotoxicity.