Fluvastatin To Inhibit Lung Cancer Cell Migration,Invasion And The Correlation With Autophagy

Objective:This study will focus specifically fluvastatin at the cellular level to inhibit invasion and metastasis, and inhibition studies fluvastatin relationship with lung adenocarcinoma cell autophagy. Thereby providing a new means for the prevention and treatment of bone metastasis, and to provide new ideas for other classes of tumor metastasis prevention. Key issues to be solved is to determine fluvastatin whether at the cellular level can prevent lung cancer cell invasion and metastasis, in this process, whether induced autophagy, using autophagy inhibitor, will affect the lung adenocarcinoma cell line invasion and metastasis.Methods:Through wound healing assay and transwell migration assay to study fluvastatin on SPC-A-1 and A549 cell migration. To study fluvastatin on SPC-A-1 and A549 cell invasion by matrigel invasion assay. Detecting autophagic bodies or other autophagy-related cellular structure. Autophagy with autophagy inhibitor activity in vitro by detecting whether the loss of fluvastatin inhibiting SPC-A-1 and A549 cell migration and invasion effect.Results:Through wound healing assay and transmembrane migration analysis experiment to determine the fluvastatin can inhibit the in vitro lung cancer SPC-A-1 and A549 cell migration.Through the basement membrane invasion assay (Matrigel invasive assay) to determine whether the fluvastatin can inhibit the in vitro lung cancer SPC-A-1 and A549 cell invasion. By electron microscopy detected fluvastatin treated lung cancer cells can be detected autophagy bodies. Using autophagy inhibitor 3-methyladenine (3-MA) and other agents inhibiting SPC-A-1 and A549 cells autophagy activity detected fluvastatin to inhibit the loss of lung cancer cell migration and invasion effect.Conclusion:Fluvastatin can inhibit lung cancer SPC-A-1 and A549 cell migration, cell invasion, and atorvastatin-treated lung cancer cells detected in autophagy bodies using autophagy inhibitor.3-methyladenine fluorine(3-MA) and other reagents inhibited SPC-a-1 and A549 cells autophagy activity detected fluvastatin to inhibit the loss of lung cancer cell migration and invasion effect.