Secondary Gelatinous Drop-like Corneal Dystrophy: Clinical, Histological And Genetic Characteristics

Part 1 Clinical characteristics of Secondary Gelatinous drop-like corneal dystrophyPurpose:To analysis the clinical characteristics of secondary gelatinous drop-like corneal dystrophy(GDLD).Methods:We retrospectively analyzed the patients in our eye center who were diagnosed with secondary gelatinous drop-like corneal dystrophy and enrolled new cases at the same time.While collecting their examine data, we had operated on the severe ones.Results:28 cases (32 eyes) attending our eye center from May 2006 to March 2016 were enrolled in this study. The mean age was 46.46±16.02 years, and was more frequent in females. Most of them (31 eyes) were induced by trichiasis, while the last one was because of alkali burn. AS-OCT showed clear images of the deposits, and the mean DT was 335.00±143.70μm, mean CT was 711.00±178.73μm. Patients who underwent operations came out a better uncorrected visual acuity(UCVA) postoperation(P<0.05).Conclusions:Secondary Gelatinous drop-like corneal dystrophy was mostly induced by trichiasis and surgical treatments could receive good therapeutic effects.Part 2 Histological and genetic characteristics of Secondary Gelatinous drop-like corneal dystrophyPurpose:To analysis histological and genetic characteristics of secondary gelatinous drop-like corneal dystrophy.Method:Corneal tissues gotten from patients underwent operations were stained with HE, Congo Red, PAS, Masson trichrome and lactoferrin. We analyzed the TACSTD2 gene of 5 GDLD patients, and compared their coding region of lactoferrin gene with 20 normal people on the other hand.Results:Congo Red and lactoferrin stain were all positive in secondary GDLD patients, while HE was 90%, PAS and Masson trichrome was 75% and 71.4% respectively. DNA sequencing showed no mutation on TACSTD2 gene, and no differences at codon 561 in exons 14 of lactoferrin gene between secondary GDLD patients and normal people.Conclusions:Congo Red and lactoferrin stain have higher specificity in diagnosing this disease. We’ve found no mutation on TACSTD2 gene, and no differences at codon 561 in exons 14 of lactoferrin gene between secondary GDLD patients and normal people.