The Relationship Between CapG And The Migration And Proliferation Of Gastric Cancer

BackgroundGastric cancer is one of the most common malignant tumors of the world, as there is no specific symptoms of early gastric cancer. At present the diagnosis rate of early gastric cancer is very low, and the treatment method of gastric cancer is very limited. In addition to the early diagnosis and treatment, postoperative chemotherapy has a great significance to improve prognosis, so finding the target of chemotherapy has become a top priority. CapG is a member of the gelsolin superfamily,widely expressed in normal tissues and tumor cells, locating in cytoplasmand nucleus. It controls the transfer, invasion and other physiological activities of the cells, and has a close relationship with the development of tumor. Many studies have shown that CapG in breast cancer, pancreatic cancer, lung cancer and other tumors can promote the migration of tumor cells,but its relationship with the proliferation ability of tumor cells remains controversial. Based on the above, this study aims to explore the relationship of CapG protein with the metastasis and proliferation ability of gastric cancer.MethodImmunofluorescence experiment located the CapG protein in BGC-823.Then lentiviral transfection silenced the CapG expression in BGC-823.Western Blot and PCR tested the expression of CapG. Using transwell assay and wound healing assay evaluated the relationship between CapG and gastric cancer. MTT assay and clone forming assay evaluated the effect of CapG on gastric cancer cells.ResultsImmunofluorescence results showed CapG protein in gastric cancer cells is distributed widely. Transwell assay and wound healing assay showed that the migration ability of silence CapG gastric cancer cell is lower than the control group decreased (P < 0.05). And the proliferation and clone forming ability of two groups have no significant difference.Conclusion1. CapG is widely distributed in the gastric cancer cells, may also exist in nuclear, further evidence is needed.2. CapG expression has an important role in promoting the migration ability of gastric cancer cells.3. Low expression of CapG does not affect the proliferation and clone forming ability of gastric cancer.