The Effects Of Placenta-specific Protein 1 On Trophoblast Cells

Background:Trophoblast cells play an important physiological role in embryo development and implantation, pregnancy induced immune tolerance, maintain normal pregnancy, and fetal growth and development stages. Trophoblast uterus interaction surface area for substance exchange to support the fetal growth. These trophoblast cells interact with the uterus and produce functional factors to modify maternal physiological systems, and render enough blood flow and nutrients to the fetal maternal interface. The progress of functional and structural changes of the placental trophoblast is strictly regulated by housekeeping genes as well as placenta specific genes.PLAC1 is a trophoblast-specific gene that maps to a locus on the X-chromosome, which is important to placental development. PLAC1 protein is restricted primarily to the differentiated tropho-blast, localizing to intracellular membranous compartment(S) in theapical region of the syncytiotrophoblast and associated with itsapical, microvillous membrane surface. PLAC1 mRNA can be detected in the pregnant women’s peripheral blood circulation in 8 week of pregnancy,22 to 40 weeks of gestation expression level did not change significantly, and be cleared at the end of termination of pregnancy. Compared with normal pregnancies, PLAC1 mRNA decreased significantly in peripheral blood of threatened abortion pregnant women. However, It increased significantly in preeclampsia patients peripheral blood. In our previous study, we also found that PLAC1 expression is significantly higher in preeclampsia patients with placenta than normal pregnancy suggesting that PLAC1 is necessary for maintaining the normal development of the placenta and fetal.Objectives:To explore whether PLAC1 involved in regulation of trophoblast cells biology function and also To study the effects of placenta-specific 1 gene on trophoblast cells of proliferation, migration, invasion and other important function investigating the possible mechanism of PLAC1 in embryonic development, placenta and function.Methods:1. The expression of PLAC1 in trophoblast cells (SWAN-71 and JAR cells) are investigated by technical studies Western blot.2. Using siRNA interference PLAC1 gene expression, CCK-8 method was used to detect the changes of cellular proliferation. Wound healing test and transwell assay were used to detect the alterations of migration and invasion.Results:1. The expression of PLAC1 was detected in trophoblast cells (SWAN-71 and JAR cells).2. Targeting siRNA interference PLAC1, SWAN-71 and JAR cells express significant inhibition the function of cellular proliferation, migration and invasion.Conclusions:The expression of PLAC1 was detected from trophoblastic cell. PLAC1 play an important physiological role in embryo development and implantation. Targeting siRNA interference PLAC1, SWAN-71 and JAR cells express significant inhibition the function of cellular proliferation, migration and invasion.