Association Between HBsAg Levels And T, B Lymphocyte Subsets Or NK Cells In Chronic HBV Infected Patients

Objective: To investigate the correlation between the levels of HBsAg and T, B and NK cells subgroup in immune incompetence(inactive phase) which were been infected by HBV. Methods: Thirty four cases of immunodeficiency(inactive phase) which were infected by HBV were selected in our study,at the same time, twenty normal cases for contorl. The levels of HBsAg was test by time-resolved fluoroimmunoassay(TRFIA). The levels of peripheral blood lymphocyte subsets and NK cells were detected by the flow cytometry. Serum HBV DNA levels was tested by Real-time fluorescent probe quantitative polymerase chain reaction(FQ-PCR). Results: The content of HBsAg was positively correlated with the percentage of B lymphocytes in inactive state cases. Spearman correlation coefficients is rs=0.37. The levels of B lymphocytes in the group of high content of HBsAg was higher than that in the healthy group as well as the group of low content of HBsAg(P<0.05). The CD3+ cell was negatively correlated with the CD56+ cell Pearson correlation coefficients is r=-0.886. However, there was no statistically significant correlation between the content of HBsAg and T, B or NK cells. Conclusions: Among patients which were been infected by HBV in inactive phase, the content of HBsAg was positively correlated with the percentage of B lymphocytes, the serum HBsAg levels could represent B cells levels to some extent. The detection of peripheral blood lymphocyte subsets and NK cells have clinical valuable for understanding the prediction of HBsAg.