The Change Of ATP-sensitive Potassium Channels Currents In Rabbits Basilar Artery Smooth Muscle Cells At Different Time Of Early Subarachnoid Hemorrhage

Objective: To investigate the changes of ATP-sensitive potassium channels’(sK_ATP) current in basilar artery（BA） smooth muscle cells（SMCS） of rabbits in early subarachnoid hemorrhage（SAH） model, using the whole-cell patch-clamp technique. Methods: Forty New Zealand White rabbits were randomly divided into sham group（C）, normal group（N）, 24hours（S1）, 48 hours（S₂） and 72hours（S₃） group after SAH（n=8, respectively）. Basilar artery smooth muscle cells（BASMCs） in each group were obtained by sequential two-step enzyme digestion.Whole-cell patch-clamp technique was applied to record cell membrane capacitance and KATP currents. Glibenclamide, a speci?c inhibitor of ATP-sensitive K+ channels was added to bath solution to identify that the outward currents were sKATP currents.With Clampfit10.0, origin8.6 and SPSS18.0 software, all data were analyzed, fitted,and plotted. All data were presented as means±SEM. Paired-samples t test or one-way ANOVA was used for statistical analysis, and values of P < 0.05 were considered statistically significant. Results: There were no significant differences in number of cell, cell size and membrane capacitance（P>0.05） in every group. Currents of sKATP in group S₁^S₃ decreased compared with group C and N. The current amplitude in group S₃ decreased most significantly（P<0.05）. There were no significant different Currents of sKATP between group C and N（P>0.05）. Conclusion: Currents of ATP-sensitive potassium channels in basilar smooth muscle cells decreased after SAH,which decreased most significantly in group S₃.