The Therapeutic Effects Of Emodin, Tea Polyphenols And Sodium Houtuyfonate On TNBS Induced Inflammatory Bowel Disease In Rats

Objective : To observe the therapeutic effects of Emodin, Tea Polyphenols and Sodium Houtuyfonate on TNBS induced inflammatory bowel disease in rat, and explore the possible immune mechanism to them.Methods:Seventy-two no specific pathogens(SPF) Sprague Dawley rats were randomly divided into nine groups, respectively, control group(A group), Emodin high dose group(B group, 50mg/kg), Emodin low dose group(C group, 25mg/kg), Tea Polyphenols high dose group(D group,340mg/kg), Tea Polyphenols low dose group(E group, 170mg/kg), Sodium Houtuyfonate high dose group(F group,100mg/kg), Sodium Houtuyfonate low dose group(G group,50mg/kg),Willow nitrogen sulfonyl pyridine group(H group, 100mg/kg) and TNBS group(I group).Colitis was induced by rectal administration of 75mg/kg of 2,4,6-trinitrobenzene sulfonic acid ethanol liquor. Control rats underwent identical procedures but were instilled with 30% ethanol. Molding after 24 hours, the B-H groups were given gavage once a day for 14 days with the dose which gave us above, TNBS model group and control group were given with physiological saline of the same volume daily. Body weights and stool state were recorded every day. Anatomyed and then abserved the general change and histologic pathological change after HE staining and scored respectively. Serum were harvested to exam the level of TNF-α, IL-lβ, IL-4, IL-10 and the content of ALT, AST, Creatinine.Result:⑴ Compared with control group, The rats of B-H groups and TNBS model group had poor general situation, decreasing weight, lower gross score and histologic pathological score, the level of TNF-α and IL-lβ were increased obviously, the expression of IL-4 and IL-10 were declined significantly, the difference was statistically significant(P<0.05). But when the group G compared with the group I, most of the index showed P > 0.05, there was no statistically significant difference.⑵ Compared with TNBS model group, B-H groups had better general situation, increasing weight, significantly reducing intestinal inflammation performance of the general situation and the histopathology manifestations, followed with the decrease of the expression of TNF-α and IL-lβ and the increase of the level of IL-4 and IL-10, and the difference was statistically significant(P<0.05).⑶ ALL the B-H groups were compared in pairs, all of the index showed P>0.05 when groups B-F compared with group H and group B compared with group C, there was no statistically significant difference. But there was statistically significant difference when group D compared with group E and group F compared with group G.⑷ Liver and kidney toxicity test, the content of ALT, AST from groups B-I were apparently higher than group A, and there was statistically significant difference bween the groups B-H and group I of the content of ALT, AST(P<0.05). But there was no statistically significant difference among groups A-I of Creatinine(P>0.05).Conclusions:⑴ Induced by the dose of 75 mg/kg TNBS liquor, the rat model of colitis were successfully established.⑵ It can reduce colon pathological tissue inflammation and injury by given gavage on rats with Emodin in the dose of 25 mg/kg and 50 mg/kg, with Tea Polyphenols in the dose of 170mg/kg and 340mg/kg, with Sodium houtuyfonate in the dose of 100mg/kg. In conclusion, these drugs in the given dose had the therapeutic effect and no liver and kidney toxicity on rats colitis.⑶ There was amount-effect relationship on Tea Polyphenols in the dose of 170mg/kg and 340mg/kg because of the therapeutic effect on rats colitis, but there was no obvious difference of the therapeutic effect on Emodin in the dose of 25mg/kg and 50mg/kg on rats colitis.