| Prevention and treatment of skeletal muscle injury has always been hotspot and difficulty in the field of sports medicine, because its inflammation has long reaction period after injury, poor quality of healing, and result in scar healing easily. Although the molecular biology in our country has made some progress, but there is no one find a solution recognized as the best to prevent and treat the skeletal muscle injury.Objective: This study of ICR mice muscle contusion after modeling, using rAAV2 mediated insulin-like growth factor-Ⅰ(IGF-Ⅰ), human umbilical cord blood mesenchymal stem cells(MSC), acute skeletal muscle contusion mice intervention, histological, biochemical studies of different angles and levels to explore gene and mesenchymal stem cells for the treatment of skeletal muscle injury and its possible mechanisms.Methods: 68 male ICR mice were randomly divided into control group(4) and experimental group(64), skeletal muscle contusion after Dayton experimental mice were divided into four groups of modeling, 16 in each experimental group was drawn according to different times, divided into injury group after three days, seven days, 14 days and 21 days. r AAV-IGF-Ⅰ group inject 40μL recombinant adeno-associated virus instantly, MSC group inject 106 cells, rAAV-IGF-Ⅰ-MSC group infect mesenchymal stem cells which was injected by adeno-associated virus-derived,natural healing group injected 40μL PBS. after injury 3d, 7d, 14 d, 21 d sacrificed animals, reserved serum and gastrocnemius.Result:(1)Recombinant adeno-associated virus-mediated iRFP713 can be widely expressed in injured muscle tissue, the control group had no expression.(2) HE staining was observed in plasma CK activity, MSC, rAAV-IGF-Ⅰ, r AAV-IGF-Ⅰ-MSC can promote muscle regeneration in mice after acute calf contusion, and reduce scarring and improve healing quality.(3) Exogenous IGF-Ⅰcan stable expression in mouse muscle, the highest grope is rAAV-IGF-Ⅰ-MSC,then rAAV-IGF-Ⅰ group, MSC group, natural healing group, sedentary control group.(4) MSC, rAAV-IGF-Ⅰ, rAAV-IGF-Ⅰ-MSC can inhibit the expression of Collagen-Ⅰ/Ⅲ m RNA, Collagen-Ⅰ mRNA expression levels low natural healing group at 7d, 14 d, 21 d,(P <0.01),, rAAV-IGF-Ⅰ group and natural healing group no significant difference at 3d(P compared to> 0.05), and the remaining two groups were significantly different(P <0.05),, rAAV-IGF-Ⅰ-MSC and MSC group also showed a significant difference at 14 days(P <0.01). 21 days, rAAV-IGF-Ⅰ group was 76% MSC group(P <0.01), rAAV-IGF-Ⅰ-MSC group was 58% MSC group(P <0.01). the treatment groups Collagen-Ⅲ mRNA expression level as compared with the natural healing group there were significant differences(P <0.01), no significant difference between groups. rAAV-IGF- Ⅰ, expression levels of rAAV-IGF-Ⅰ-MSC two groups of Collagen-Ⅲ mRNA lower than the natural healing group at 21 days(P <0.01).Conclusions:(1) MSC and IGF-Ⅰ injury have therapeutic effect of skeletal muscle(2) MSC have faster treatment for skeletal muscle injury, but it has short duration(3) rAAV2 mediated IGF-Ⅰ has slower reaction time for muscle injury time is slower, but the long duration of action(4) rAAV-IGF-Ⅰ-MSC treated group is superior to simple MSC and rAAV-IGF-Ⅰ group. |
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