| Background:We examine the expression and clinical significance of SIAH2 in gastric cancer tissues. Meanwhile, we transfect SIAH2 siRNA inhibitor to gastric cell lines, investigating the effects of transfection on cell invasion and metastasis.Methods:We collect human gastric cancer samples and the corresponding adjacent non-tumor tissues and detect SIAH2 expression by immunohistochemistry. Meanwhile, we detected SIAH2 expression levels by quantitative real-time PCR(qRT-PCR) in several gastric cell lines. The gastric cell line AGS, which had the highest level of SIAH2 expression, was transfected with siRNA inhibitor to down-regulate the expression of SIAH2. Cell migration was performed using wound-healing method and cell invasion was measured in transwell chambers.Results: The expression level of SIAH2 was higher in gastric cancer tussues than in NATS. The expression level of SIAH2 was associated with advanced clinical tumor stage, tumor size and lymph node metastasis. The transfection was confirmed by afluorescence microscope and flow cytometry. The expression of SIAH2 in the gastric cancer cell line AGS, was confirmed being down-regulated after transfected with siRNA inhibitor. The down-regulating of AGS cells also caused the inhibition of the migration and invasion of the gastric tumor cells.Conclusion: SIAH2 plays a critical role in the migration and invasion of gastric tumor cells. SIAH2 may serve as a prognostic marker or a potential target for gene therapy. |
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