Related Research Of A DMOG-loaded Composite Of Silk Fibroin/Octacalcium Phosphate For Bone Repair

Objective: Bone defect caused by different kinds of orthopedic disease brings huge pain to patients and has great impact on patients’ family and the society. In order to solve this problem, bone repair materials are needed. In this study, a new DMOG-loaded composite material, silk fibroin/octacalcium phosphate, is designed for bone regeneration. The physical and chemical properties, biological properties and bone formation capability of the composite biomaterials were investigated in this study.Methods: 1. The silk fibroin and octacalcium phosphate were prepared with raw materials, then silk fibroin/octacalcium phosphate composite scaffolds loading DMOG were constructed. The obtained silk fibroin/octacalcium phosphate composite were characterized by fourier transform infrared spectroscopy(FTIR), X-ray diffraction(XRD), transmission electron microscopy(TEM), scanning electron microscope(SEM). The thermogravimetric analysis, mineralized experiment, mechanical testing, washout resistance and drug release of DMOG of the silk fibroin/octacalcium phosphate porous scaffolds were also carried out. 2. MC3T3-E1 cells from mice were cultured on the materials after sterilized by radiation, the biocompatibility was determined by CCK-8 assay and cytoskeleton staining. 3. Twelve male SD rats were used in the animal experiment, these scaffolds were implanted in skull defect of the rats. After 6 and 12 weeks, the rats were sacrificed to obtain the samples for micro-CT and histological evaluation.Results: 1.The results of FTIR, XRD, TGA, washout resistance and SEM proved that stable silk fibroin/octacalcium phosphate composite scaffolds with high OCP content were successfully prepared in this research, and DMOG could be incorporated in these scaffolds. TEM showed that the crystal size of OCP became smaller in the silk fibroin/octacalcium phosphate composite compared with pure octacalcium phosphate. The results of mineralized experiment and mechanical testing demonstrated the scaffolds having certain mechanical strength, and could translate into hydroxyapatite. The drug release experiment indicated that the drug released steadily. 2. Cells on the composite materials incorporating DMOG all showed proliferation, and no difference was observed in cell morphology. 3. From the animal experimental results, it could be seen that the DMOG-loaded SF/OCP composite scaffolds had the better bone formation ability, but the sample size is small.Conclusion: The stable SF/OCP composite scaffolds with well bioactivity were successfully prepared, the DMOG could be encapsulated in the scaffolds and slowly released, and the materials could induce the formation of hydroxyapatite. These biomaterials showed low cytotoxicity, good biocompatibility and good osteogenesis capability, which may be a promising biomaterials for bone regeneration.