| Background:Gastric Cancer is a common malignant tumor of digestive tract. But it is difficult to diagnose due to the unclear development mechanism at present. MicroRNAs (miRNA) is a kind of newly found micromolecule non-coding RNA which plays an important role in regulating cell growth, differentiation, proliferation, apoptosis as well as cancer occurrence and development. This study will focus on the relationship between the expression of miR-630 in gastric cancer tissue and clinicopathologic characteristics, and the role of miR-630 in gastric cancer cell proliferation, cell apotosis. and so on.Methods:1. Gastric cancer and adjacent specimens from 40 patients (wo had not received neoadjuvant chemotherapy) were collected. The expression level of miR-630 was investigated by quantitative real-time polymerase chain reaction (qRT-PCR).and its association with tumor clinicopathologic feature was analysed.2. MiR-630 mimics and inhibitor was transfected into gastric cancer cell HGC-27 and HGC-803, mimics control and inhibitor control were used as the negative control. CCK-8 kit was used to detect the effect of miR-630 on the proliferation of gastric cancer cell HGC-27 and MGC-803. FCM was used to detect the effect of miR-630 on apoptosis of gastric cancer cell HGC-27 and MGC-803.Results:1.The quantitative real-time PCR was sensitive and specific in miR-630 detection. The expression of miR-630 in gastric cancer tissues(0.85±0.24) was significantly down regulated than that in normal gastric tissues(1.63±1.02) (P<0.05), significantly lower in gastric cancer tissues with lymph node metastases(0.65±0.13) than that in those without lymph node metastases(0.94±0.48) (P<0.05), and significantly higher in cases of undifferentiated cancer as compared to those of highly differentiated cancer(P<0.05). However it was not significantly associated with gender and age (P>0.05).2.Cell proliferation assay results reveal that miR-630 could decrease the proliferation of the gastric cancer cell HGC-27 and MGC-803; the flow cytometry results indicated that miR-630 could increase the cell apoptosis of the gastric cancer cell HGC-27 and MGC-803.Conclusion:The expression level of miR-630 decreases obviously in gastric cancer tissue and externally expressed miR-630 can inhibit gastric cells proliferation and facilitate its apoposis. The experiment shows that as a potential cancer inhibitor, miR-630 can play a role of inhibting cancer. It provides a new experimental basis for exploring new effective diagnosis and treatment of gastric cancer. |
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