Effect Of Exogenous Thyroxine On The Alcohol-Related Neurodevolpmental Disorder In Rat

Objective:To investigate the effect of exogenous thyroxine on the Alcohol-related Neurodevelopmental Disorder during the period of fetal alcohol exposure in rat.Method:The normal pregnancy (group N) and alcohol exposure rats model were established; and the alcohol exposure groups were divided into alcohol group(group A), alcohol+thyroxine group(group A+T), and the surrogate mother group(group C). The body weight and blood thyroxine contents were measured at different postnatal periods in the neonatal rats. The expression of BDNF in Purkinje cells of cerebellum was determined by immunohistochemical staining method. The BDNF contents of brain tissue at different postnatal periods was measured by ELISA method.Results:1) The Body weights and the thyroxine levels were significantly higher on different periods in the group A+T and group N than that the group A (All p<0.01). 2)The BDNF positive of Purkinje cells were observed in the rats’cerebellar cortex on the 7th day after the birth; the number of BDNF positive Purkinje cells was more in the group A+T and group N, and more orderly arrangement, longer projection and some visible branches on the 28th day after the birth; 3)the BDNF contents were significantly higher on various periods in the group A+T and group N than those the group A (p< 0.01);the BDNF content of the brain was higher in the group A+T than that the group N (p< 0.01) on the 20th and 28th days after birth.Conclusion:The thyroxine injection can improve the weight loss and low blood thyroxine in the alcohol exposed newborn rats during the fetal period, which is able to promote the synthesis of BDNF in the brain of early postnatal rats and contribute to the development of BDNF positive neurons, so as to eventually improve the neurological developmental disorder during the period of fetal alcohol exposure.