Association Of Methylenetetrahydrofolate Reductase (MTHFR) C677T Gene Polymorphism As A Risk Factor For The Diabetic Nephropathy In Type 2 Diabetes Mellitus

Objective:The aim of the study was to explore the association of the methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism as a risk factor for the development of diabetic nephropathy in type 2 diabetes mellitus.Methods:According to WHO 1999 criteria,220 T2DM patients were selected where(male 120 cases,female 100 cases,including diabetic nephnopathy)from the medical ward of Endocrinology department during December 2013 to December 2015.The 115 control group were selected as healthy individual in the outpatient department(male 60 cases,female 55 cases),and the clinical data were collected.Genomic DNA was extracted from peripheral blood leucocytes of the participants. The MTHFR polymorphism was screened by means of PCR-(Hinfl) in all subjects and identification of the mutation directly by sequencing the PCR production. At last we compared the difference of frequency in allele and genotype among groups. Statistical analysis was performed by SPSS 21.0 version.OR,CI95% calculated,and results were expressed as P value where statistical significance was set at(P<0.05)Results:1.The frequency of the MHTFR mutation was stalistically significant between the control and diabetic group.(P<0.05).2.The frequency of the MHTFR mutation was statistically significant between non diabetic nephropathy group and diabetic nephropathy group.(P<0.05)3.The tendency value between the nondiabetic nephropathy and diabetic nephropathy group was statistically significant.Conclusion:1.The methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism have been associated with type 2 diabetes or diabetic nephropathy.2. Allele T is the risk factor of type 2 diabetes or diabetic nephropathy.3. Triglyceride, creatinine, BUN, high fasting blood glucose, and hypertension are independent risk factors for diabetic nephropathy.