Analysis Of Related Factors Of The Outcomes Of The Vitrified Frozen- Thawed Transfer In Hrt-Fet

Objective:To investigate the factors influencing the clinical outcomes of the vitrified frozen-thawed embryo transfer which choose hormone replacement therapy preparing the endometrium without pituitary down-regulation, in order to improve clinical outcome.Methods:This is a retrospective study analyzing 305 first-time HRT-FET without pituitary down-regulation cycles only included embryos in cleavage stage by vitrification technique. The data was gathered from the infertility couples who received treatment from January 1st in 2014 to November 31st in 2015 in Guangxi Center for Reproductive Medicine of The First Affiliated Hospital of Guangxi Medical University. The data was d divided into two groups according to clinical pregnancy or not. There were 120 cycles in clinical pregnancy group and 185 cycles in no clinical pregnancy group. Analyzing a number of factors, including the age of female, female body mass index (BMI), basic follicle-stimulating hormone(FSH), classification and duration of female sterility female sterility, time of endometrium translation, endometrium thickness in the day of embryo transferred, the number of embryo and high qualified embryo transferred per cycle and the choice of fertilization mode, to find which one can impact the clinical pregnancy. The quantitative data was compared and analyzed by independent-samples T test and independent-samples rank-sum test. And the one belongs to enumeration data was compared and analyzed by chi-square test. Correlative factors of clinical pregnancy were finally analyzed by binary logistic regression analysis.Results:1. Among the 305 FET cycles, a total of 612 embryos were transplanted, and average transferred embryos were 2.0±0.5 per cycle. Following the 612 embryos in 305 cycles,141 embryos were implanted, the implantation rate is 23.0%. And 120 couples were pregnancy. The overall pregnancy rate per cycle was 39.3%.2. Result of factors influencing the clinical pregnancy in HRT-FET by single factor analysis was that there was no significant difference of female BMI, basic FSH, classification and duration of female sterility female sterility, time of endometrium translation and the choice of fertilization mode (P> 0.05).3. Result of factors influencing the clinical pregnancy in HRT-FET by binary logistic regression analysis was that there was a significantly positive correlation between the number of high qualified embryo transferred per cycle and clinical pregnancy (B=0.614, OR=1.204).Besides, endometrium thickness in the day of embryo transferred was also a significant positive correlation with clinical pregnancy. There was no significantly correlation between the rest of the factors, such as the age of female and the number of embryo transferred, and the clinical pregnancy(P> 0.05).4. There was no significant difference in the factors of the number of high qualified embryo transferred with clinical pregnancy if the endometrium thickness in the day of embryo transferred was less than 9.0mm (P>0.05). The result revealed that the more high qualified embryos was transferred, higher the clinical pregnancy rate was, when the endometrium thickness in the day of embryo transferred in these cycle ranged between 9.0mm and 12.0mm.The difference was statistical significance(P<0.05).Conclusions:1. The clinical pregnancy rate can be enhanced with high qualified embryos and appropriate endometrium thickness in the day of embryo transferred in HRT-FET cycles.2.In HRT-FET cycles, if the endometrium thickness in the day of embryo transferred in these cycle is suitable, the more high qualified embryos is transferred, higher the clinical pregnancy rate is.3.The clinical pregnancy in HRT-FET cycle is not affected by the length of endometrium translation time. In clinical work, it can be decided by actually need.4.It is not the key factors influencing clinical pregnancy in HRT-FET cycle, including the age of female, female BMI, basic FSH, classification and duration of female sterility, the number of embryo transferred per cycle and the choice of fertilization mode.