Expression And Significance Of ER And PR In Uterine Leiomyoma And Smooth Muscle During Pregnancy

ObjectiveTo research the expression of ER(estrogen receptor) and PR(progesterone receptor) in pregnant women’s uterine leiomyoma and normal smooth muscle, and the vomule changes of uterine leiomyomas during pregnancy, and then to explore the possible association between ER and PR and the growth of uterine leiomyoma during pregnancy.Methods1.Immunohistochemistry experiment:we collected 9 pregnant women’s uterine leiomyoma(UtLM) and corresponding normal smooth muscle(UtSM), who did cesarean section and hysteromyomectomy at the same time coming from Qilu Hospital, Shandong University. Simultaneously, we collected 12 non-pregnant women’s uterine leiomyoma and corresponding normal smooth muscle at corresponding time period, who did hysteromyomectomy or hysterectomy due to uterine leiomyoma. Immunohistochemistry was used to detect the expression of ER and PR in these tissues.2.The vomule changes of uterine leiomyomas during pregnancy:we choosed 110 pregnant women with uterine leiomyoma as the research objects, who came from Qilu Hospital, Shandong University, and recorded the sizes of uterine leiomyomas through ultrasonic reports in early pregnancy (less than 13 weeks), mid pregnancy(16 to 23 weeks gestation) and late pregnancy(37 to 40 weeks gestation).Resultsl.The expression of ER in uterine leiomyoma and smooth muscle during pregnancy and non-pregnancy was detected by immunohistochemical SP method. The results show that the expression of ER in pregnant women’s uterine leiomyoma and corresponding smooth muscle was negative(P>0.05). The expression of ER in pregnant women’s uterine leiomyoma was significantly lower than that in non-pregnant women’s uterine leiomyoma(P<0.01).2.The expression of PR in uterine leiomyoma and smooth muscle during pregnancy and non-pregnancy was detected by immunohistochemical SP method. The results show that the expression of PR in pregnant women’s uterine leiomyoma was significantly higher than that in corresponding smooth muscle(P<0.05). The expression of PR in pregnant women’s uterine leiomyoma was significantly lower than that in non-pregnant women’s uterine leiomyoma(P<0.01).3.The volume changes of uterine leiomyomas during pregnancy. The results show that there was no significant difference in the sizes of uterine leiomyomas between early mid and late pregnancy(P>0.05). But uterine leiomyoma had an increasing trend in early and mid pregnancy and had a decreasing trend in late pregnancy.ConclusionsThe expression of ER and PR in pregnant women’s uterine leiomyoma was significantly lower than that in non-pregnant women’s uterine leiomyoma which indicates that ER and PR may play an important role in the growth of uterine leiomyoma during pregnancy. However, whether ER deletion is related to the slow growth of uterine leiomyoma with high E2(17beta-estradiol) and P4(progesterone) during pregnancy still remains unclear.