| Background:With the rapid development of the domestic economic construction, increasing living standards and transportation industry development, aging population problem is getting worse. The incidence of osteoporosis and fractures caused by osteoporosis is increasing continuously and become a common disease in clinical department of orthopedics. Patients suffer from osteoporosis and fractures caused by osteoporosis. Because of long course of disease and the obvious clinical symptoms, patients and their families suffered heavy financial burden and the society is under severe pressure.Osteoporosis is a metabolic bone disease, the cause of osteoporosis is more complicated, and the etiological mechanism is not clear up to now. Presently the research results of osteoporosis etiological mechanism as follows:First, genetic factors. VDR (Vitamin D receptor) gene is associated with bone mineral density, vitamin D plays an important regulatory role on bone metabolism. Studies have shown that the BMD of patients was significantly reduced dueing to TGF-β gene mutant, Secondly, nutrition and exercise factors. The elderly have impaired digestion and tend to lack of protein, calcium, trace elements, etc. In addition, less exercise is a important factor which lead to osteoporosis. Thirdly, endocrine factors. Endocrine factors includ sex hormone, calcitonin and adipokines, etc. Estrogen receptors in human osteoblasts and osteoclasts has been confirmed. Estrogen receptor bind osteoblasts and osteoclasts cells, which can preventing bone resorption. Osteoclasts has calcitonin receptors. It’s combination with calcitonin can inhibit cell activity. Recent studies have shown that adipose tissue can secrete a variety of cytokines called adipokines. including chemerin, leptin, adiponectin,resistin, etc. Adipokines are not only involved in energy metabolism, lipid metabolism, but also involved in inflammation and immune response, Adipokines play a important role in bone metabolism. The bone loss is often accompanied by increased bone marrow fat content. Adipose tissue in bone plays an important role in the formation of bone marrow and hematopoietic. Chemerin receptor and Chemerin has been found, its role in the treatment of osteoporosis gradually attracts more and more attention.Objective:By establishing the model of osteoporosis and femoral fracture in rats, we provide the experimental basis for Chemerin and osteoporosis and fractures. By grouping controlled, the concentration of different time determination of Chemerin in rat serum, we can understand the relationship between Chemerin and osteoporosis, fracture.If it is proved that the pathogenesis of Chemerin on osteoporosis and fracture recovery regulation, for further study on the specific regulatory mechanism and treatment of osteoporosis and promote fracture healing up,we maybe find a new treatment method.Methods:A total of 30 female Wistar rats, weighing 120-170g. The rats were randomly divided into five groups,6 rats in each group. Group A is sham operation (SHAM) group. B group is ovariectomied group (Ovariectomy, OVX). C group is ovariectomied and Estrogen therapy group (OVX+E2).Group D is ovariectomized and femoral fracture group (OVX+FF).Group E is femoral fractures group (femoral fractures, FF).The establishment of Osteoporosis model is using ovariectomized method.After the osteoporosis model was established successfully three months later, and then closed femoral fracture model is established in group D and E. The Chemerin of serum concentrations in group A and B were detected at the same time. After the establishment of the femur fracture model, the Chemerin of serum concentrations were detected in group A,B,D,E respectively 1 week,2 weeks,4 weeks. After the establishment of osteoporosis model, Group C was given estrogen 5ug, every day for 6 weeks, then Chemerin of serum concentrations were detected. Detection method is used enzyme linked immunosorbent assay (ELISA). Analysis of serum Chemerin concentration.Results:The serum Chemerin concentration difference between group B and C was not significant(P>0.05) after given estrogen in group C six weeks;The difference of BMD in osteoporosis group C was significantly higher than that of group B.The difference was statistically significant(P<0.05);The serum Chemerin concentration in osteoporosis group B was significantly higher than that of group A. The difference was statistically significant (P<0.05); The serum Chemerin concentration in group E was significantly higher than that of group A.The difference was statistically significant(P<0.05);The serum Chemerin concentration in group D was significantly higher than that in group B.The difference was statistically significant(P<0.05).Conclusion:The results reveal that estrogen has no influence on Chemerin concentration. Estrogen has influence on Osteoporosis’s pathophysiology. Chemerin is involved in the pathophysiology of osteoporosis. Chemerin is involved in the fracture repair process. |
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