Effect And Mechanism Of Benzopyrene On Migration And Invasion Of Lung Cancer Cells

Objective:To investigate the potential roles of tobacco sourced B[a]P and NNK on cell metastasis and invasion and to assess its underlying mechanism.Methods:Effects of B[a]P and NNK on A549 cell proliferation, metastasis and invasion were analyzed using MTT assay, Transwell assay and scratch method respectively. Besides, effects of tobacco sourced carcinogen on cytokines and chemokines secretion were detected using ELISA. Moreover, correlation between cytokines and chemokines expression and cancer cell migration and invasion were assessed using gene silencing method.Results:Data showed that both B[a]P and NNK either at high or low dose performed no significant difference on A549 cell proliferation with time increasing. NNK performed no significant difference on A549 cell migration and invasion, however, B[a]P significantly increased A549 cell migration and invasion compared to the control group (P<0.05). Consequently, except for IL-6, the secretion of IL-8, CCL-2 and CCL-3 were significantly increased by B[a]P treatment compared to the control (P<0.05). Furthermore, when CCL-2 and CCL-3 were silenced, the migrated and invasive A549 cells were significantly decreased compare to the control respectively (P＜0.05), while silenced IL-8 drastically decreased the migrated and invasive cells compared to the control (P<0.01).Conclusion:The data presented in this study illustrated that there may be significant correlation between smoking and lung cancer metastasis. B[a]P maybe an excellent contributor for lung cancer metastasis through up-regulating IL-8, CCL-2 and CCL-3 expression.