The Experimental Study On Immune Response Of Pulmonary Fibrosis Through Intervention Of Notch Signaling Pathway By Yangfei Huoxue Decoction

Pulmonary fibrosis is a complex pathophysiological process that most of interstitial lung disease have common final outcome, and its pathological feature is diffuse chronic inflammation of alveolar wall (including respiratory bronchioles) and interstitial fibrosis, eventually loss of respiratory function because of lung parenchyma is gradually replaced by fibrous scar tissue to replace. With the pollution, environmental degradation and other factors, pulmonary fibrosis has become a serious health problem, due to the limited treatment of Western medicine, traditional Chinese medicine treatment of pulmonary fibrosis has become a hot research topic. This topic under the guidance of collateral disease, with Supplementing Qi and nourishing Yin, Promoting blood circulation to remove obstruction as the main treatment method, selected clinical prescription "Yangfei Huoxue Decoction", start point of immune response of pulmonary fibrosis through intervention of Notch Signaling Pathway, from three part that the literature, theory and experiment to ran a more systematic and in-depth study.The first part, literature research. Respectively, from the two parts of Western medicine and traditional Chinese medicine to clarify the pulmonary fibrosis. Western medicine think that pulmonary fibrosis is mainly caused by the two major categories of lung disease (clear cause and unknown cause), its pathogenesis is closely related to the cellular immune response mediated by various cytokines, including Thl/Th2 cytokine imbalance development plays an important role in pulmonary fibrosis. At present, western medicine for the treatment of this disease is poor, can not effectively solve the pain of patients, and side effects, however traditional Chinese medicine treatment can improve the clinical symptoms of patients with pulmonary fibrosis. On this foundation, to describes the understanding and treatment of pulmonary fibrosis throughout the ancient and modern Chinese Medicine from the aspects of the name of disease, etiology and pathogenesis, Chinese medicine treatment, and from the perspective of intervention of pulmonary fibrosis by Notch signaling pathway in the immune response, to analyses and discusses the mechanism for the occurrence and development of pulmonary fibrosis.The second part, theoretical research. Based on the theory of Ye Tianshi that "Disease progressively, Chronic Diseases Transforming to Collaterals", combining with the characteristics of lung physiology pathology, indicates that the pathogenesis of pulmonary fibrosis has the characteristics of disease progressively, from functional to organic disease lesions. The pathogenesis of this disease in the early course is Qi disorders and dysfunctiong. In the late course of later disease, the main pathogenesis is pulmonary deficiency can not run support organs and blood stasis, so tonifying Deficiency and removing obstruction in the channels is important for the treatment of therapies of pulmonary fibrosis.The third part, experimental study. Useing the method for replication of rat pulmonary fibrosis model by single intratracheal instillation of bleomycin, from the perspective of Notch signaling pathway and immune regulation to observe the effect of Yangfei Huoxue decoction on rats with pulmonary fibrosis. After the establishment of the model rat lung tissue infiltration of inflammatory cells and hyperplasia of fibrous connective tissue, lung cell structure turbulence, to Yangfei Huoxue decoction treatment can significantly improve lung inflammation of rats, and reduce the degree of pulmonary fibrosis. The lung tissue of rats after modeling appeared a large number of inflammatory cell infiltration, hyperplasia of fibrous connective tissue and lung cell structure disorder, after the treatment of Yangfei Huoxue decoction can obviously improve the lung tissue inflammation and reduce the degree of pulmonary fibrosis. At the same time, it is observed that this decoction can regulate the DLL1/Notch2, DLL1/Notch3 signaling pathway, affect the drift of Thl/Th2, and can significantly reduce the expression of CD80, CD86, CD28, IL-6, TNF-a. It showed that the costimulatory molecules and related cytokines also had intervention effects on the pulmonary fibrosis. The results showed that the mechanism of pulmonary fibrosis by the treatment of Yangfei Huoxue Decoction may be related to the influence of the immune response.Through the above research, discusses the mechanism of treatment of pulmonary fibrosis from two parts that the Yangfei Huoxue Decoction intervention Notch signal pathway and affect the immune regulation. These all can provide a reliable theoretical and experimental basis for traditional Chinese medicine to clinical treatment of pulmonary fibrosis.