| Objective:The acute toxicity test, the ear swelling model, Glacial acetic acid-induced writhing response model, and the rat’s cervical spondylotic myelopathy model were applied to study the safety, the anti-inflammatory and analgesic effects, and promotions in the recovery of the spinal cord function after chronic oppression of the prescription for nourishing qi and activating blood (PNQAB), respectively. The mechanisms of the PNQAB in promoting the spinal cord function recovery have been illustrated, providing the clinical application of the PNQAB with a better theoretical basis and practices guidance.Methods:1. Twenty ICR mice were randomly selected,5 mice were used for the control group, the rest for the experimental group, then the method of maximum dosage was applied to estimate the acute toxicity of the PNQAB; 2. The xylene-induced ear swelling model and Glacial acetic acid-induced writhing response model were utilized to study the anti-inflammatory and the analgesic effects of different concentrations PNQAB on mice; 3. Ten SD-Rats are used as the sham-operated group in this experiment selected randomly from the sixty subjects, fed with the normal saline within explosion of the vertebral lamina and free from oppression of the spinal cord; the remaining are applied to establish the rat’s spinal oppression model by grafting the self 7th cervical vertebrae spinous process at the part of C4-5 or C5-6 intervertebral space, then divided into three groups based on the assessment results of the spinal cord function at the 1st day postoperatively, feeding with the PNQAB under different concentrations (Group A), Jinfukang (Group B) and Normal saline (Group C), respectively. All the animals are gastric perfusion 4 weeks, the movement functions were observed according to the inclined test, Tralov score, BBB ratings at the beginning of the gastric perfusion,1 weeks,2 weeks,3 weeks,4 weeks. Then all the animals are sacrificed by the end of the gastric perfusion and the damaged tissue of spinal cord have been removed for the examination of morphological changes (after stained with HE) and the determinations of contents of SOD, MDA, PGE2 and PLA2 (tested by ELISA method) of the specific tissue in rats’ spinal cord.Result:(1) The daily maximum dosage equal to crude drugs to mice is 892.5g·kg-1,315 times of daily clinical dosage of adult.under the dose, obvious toxic effects is not seen, no animals died within 7 days. (2) It is found that PNQAB under 3 concentrations inhibit the degree of ear swelling and decrease the times of writhing response in mouse significantly. (3) Behavior observation:PNQAB under different concentrations as well as Jingfukang particles greatly increase average degree in inclined test, Tralov score, BBB ratings. Compared with NS group, its effect is significant(P<0.05). (4) HE staining:The results of HE staining show that glial scars and cavitation are sporadically formatted in Group A, Group B, but existed widely in Group C; and the total areas of cavitation in Group A and Group B are obviously less than Group C. (5) ELISA:The groups fed with the PNQAB under high/medium concentration and Jinfukang can notably decreased the content of PGE2(P<0.05), while all the groups fed with the PNQAB and Jinfukang have a positive effects on the inhibition of PLA2 production in the tissues of the rats’ damaged spinal cord significantly(P<0.05), compared to fed with normal saline; The PNQAB regardless of the concentration difference and Jingfukang particles can both increase the content of SOD in rats’ damaged spinal cord tissues significantly(P<0.05); while the PNQAB under high/medium concentrations and Jingfukang particles can also notably decrease PGE2、 MDA content, but not for the PNQAB in low concentration.Conclusion:The PNQAB is safe and has the anti-inflammatory and analgesic effects, it can effectively enhances the scavenging rate of the reactive oxygen radicals and inhibits inflammatory response in tissues of the damaged spinal cord to promotes the recovery of the spinal cord function. |
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