The Molecular Mechanism Involved In The Effects Of HMGB1 On Mesenchymal Stem Cell Osteoblastic Differentiation And Migration

The migration of mesenchymal stem cells (MSC) and osteogenic differentiation play an important role in fracture healing. High mobility group box 1 (HMGB1), a widely distributed inflammatory factor in fractures, has been confirmed to act as a chemoattractant to MSC. To investigate the effect of HMGB1 on MSC osteogenic differentiation and migration and the underlying mechanism, we evaluated the synthesis of MSC chemokines and the consequent activation of signaling pathways following HMGB1 stimulation.We use Alizarin red staining and ALP staining to prove the osteogenic differentiation. And use test the migration by scratch assay. To detect cytokine secretion, antibody array assays were performed. These results suggest that HMGB1 enhances the secretion of multiple cytokines by MSC and promotes biological functions through the signaling pathway. Then we use the western-blot and inhibitor to test the function of signaling pathway more deeply.These results suggest that HMGB1 induce the cytokine secretion of MSC. And the cytokines promote the signaling pathway. At last, the biological functions of MSC has been promoted through the signaling pathway. This study may provide a theoretical basis for the development of new techniques for the treatment of fractures in the future.