Effects Of ROS In Chronic Renal Injury

Oxidative stress plays an important role in the stabilization of human physiological function and the developing of diseases. Reactive oxygen species (ROS) over produced and/or the insufficient eliminating of ROS are the important factors to the imbalance of vascular homeostasis and vascular remodeling, participating in hypertensive nephropathy characterized by damaged myogenic response. However, the mechanism of how the O2- and H2O2 affect the vascular homeostasis is still unclear. Thus, to investigate the mechanism of O2- and H2O2 in renal afferent arteriolar dysfunction is quite significant.Chronic renal damage causes renal inadequacy, Glomerular filtration rate decrease, the blood volume and pressure increase. Hypertension and chronic kidney diseases are the main risk factors for cardiovascular disease. The function damages of small resistant vessels are the basic pathological changes of hypertension.5/6 Renal reduced mass (RRM) with high salt intake in C57B1/6 mice was used for built up chronic renal damage animal model for the mechanism studies of chronic renal damages and renal dysfunction.Objective:the study is aimed of a further understanding in the mechanism of ROS in chronic renal damage.Methods:We used C57B1/6 mice with 5/6 RRM with high salt diet to make a chronic kidney injury model, to investigate and analysis the renal pathological changes in mice plasma BUN, Creatinine, urinary sodium and potassium, urinary protein, urinary creatinine and 8-Isoprostane, to measure blood pressure, serum creatinine and creatinine clearance rate, etc. Immuno-histological methods were used for observing the transforming growth factor beta (TGF beta), fibronectin (FN) and type IV collagen (collagen IV, col IV) expression in the kidneys of sham, RRM and RRM with tempol groups.Results:we made the chronic renal injury animal model by 5/6 renal reduced mass (RRM) with high salt intake in C57B1/6 mice. Our results showed that the urinary 8-Isoprostane increased, urinary albumin excretion increased, serum creatinine increased, and the volume of glomerulus increased, meanwhile the glomerular filtration rate decreased in the RRM with high salt diets group. It was evidences that ROS played an important role of the chronic renal injury in mice. However, in the RRM with high salt intake and with tempol treated group, we show that the urinary 8-Isoprostane and albumin excretion recovered, serum creatinine normalized, and the glomerular filtration rate increased compared with RRM with high salt diets group. Antioxidant treatment is benefit for the chronic renal injury recovery.Conclusion:ROS plays an important role in the chronic renal injury and the process of chronic renal injury. Antioxidant treatment is a good way and benefit for the chronic renal injury recovery.