Apoptosis Of CT26 Tumors Affects On Immune Cells In Mice

Objective To investigate the apoptosis in colorectal CT26 cells on mouse T lymphocytes and natural killer(NK) cells and macrophages affect cell proliferation and activity and to explore its possible mechanism.Methods Each group( apoptotic cells, necrotic cells and normal cells in the logarithmic growth phase) CT26 colorectal cancer cells and mouse cytotoxic T lymphocyte(CTL), NK cells and macrophages cultured together.Then using 51 Cr release experimentally determined cell activity. CCK-8 was detected in peripheral blood NK cells, CD8+ T lymphocyte proliferation and macrophage.ELISA was detected the CT26 cells soluble Fas(sFas) levels. Even further, we also observed the expression of inflammatory factors like IL-4, IL-10, IL-12,IFN-γand TGF-βby enzyme-linked immunosorbent assay.Results 51 Cr release assay results are shown that cultured with apoptosis in colorectal CT26 CTL, NK cells and macrophage activity was inhibited. Test results by CCK-8 showed apoptosis in colorectal CT26 cells can inhibit NK cells in mice, CD8 + T lymphocytes and macrophages proliferation. ELISA testresults showed that apoptosis CT26 sFas levels were significantly higher than other groups.Conclusion Apoptosis in colorectal CT26 cells could inhibit the proliferation and activity of CTL, NK cells and macrophages in mice.