The Preparation Of Silk Fibroin(SF)-Based Composite Porous Scaffolds For Application In Bone Tissue Engineering

The case of bone defect resulted from congenial dysplasia, tissue pathological changes and trauma are very common, especially the bone tissue pathological changes happened to the old, for example the osteoporosis. The bone substitute prepared by so far fail to own great mechanical properties, secondary formability and biological properties at the same time. The silk fibroin were used as the organic templates, we have preparated the silk fibroin/cellulose whiskers/chitosan composite porous scaffolds with different self-assembled layers and the grapheme oxide/hydroxyapatite/silk fibroin porous composite scaffolds with different mineralization. The porous test, SEM, FTIR, XRD, mechanics performance were used to character the SF/CNW/CS composite scaffold. The Result showed that the SF/CNW/CS composite scaffold presents a hierarchical lamellar construct, and the lamellar thickness in the SF/CNW-CS composite scaffold increased with increase in the numbers of self-assembled layers but presented a simultaneously decreased porosity. Through biological performance tests show that pure SF, stents, SF/CNW/CS composite porous scaffold all have good blood phase solution and high protein adsorption capacity, and the SF/CNW/CS composite porous scaffold is more helpful to cell proliferation and activity than the pure SF scaffold. The XPS, SEM, TE-SEM, FTIR, XRD, porous test and mechanics performance were used to character the c GO/HAP/SFcomposite scaffold. The result indicated that: the c GO/HAP particle has high crystallinity, the HAP particles showed a flexible and thin strip structure, and disordered tiled on both sides of the c GO layers. The density and porosity of c GO/HAP/SF composite scaffold all show a step-by-step structure. With the increase of mineralization, the mechanical performance of c GO/HAP/SF composite scaffold enhanced gradually, the breaking strength, the fracture strain and initial modulus of c GO/HAP/SF composite scaffold materials were retained at most degree when the c GO/HAPwas 1:4. The cells were cultured on the craft out of pure SF scaffold, HAP/SF scaffold and c GO/HAP/SF scaffold to study their biological properties. The Protein adsorptivity, Hemocompatibility, fluorescence microscope, MTT assay, alkaline phosphate and osteocalcin and RT-PCRwere used to study the cell proliferation and activity. The result indicated that: pure SF scaffold, HAP/SF scaffold and c GO/HAP/SF scaffold all have high blood phase solution and high protein adsorption capacity. And the protein adsorption ability of c GO/HAP/SF composite scaffold is best. The indexes of cells’ proliferation and cells’ competence of forming bone of the c GO/HAP/SFcomposite scaffold were higher than the SF scaffold and HAP/SF scaffold.