| Objective:This study was on the basis of cerebral ischemia/reperfusion(I/R) model by SD rats Combining with pathology, molecular biology and drug intervention. Discussed the PDCD-5 expression changes in cerebral ischemia/reperfusion rat’hippocampus cells, and the expression of Edaravone injection after the intervention. Preliminary discussion the PDCD-5’ role in the nerve protection and the protective effect of Edaravone-injection. Method:Prepare 80 healthy male SD rats which weight between 250g-300 g, then randomly divided into four groups: normal group(n = 8),Sham-operated group(n = 24), cerebral ischemia/ reperfusion( I/R)(n = 24)and butylphthalide intervention group(n =24). Both of the later two groups were randomly divided into four subgroups( 6 rats for each subgroup):6 hours group(6h), 1 day group(1d), 3 days group(3d) and 7 days group(7d). Select Longa method to create SD ischemia/reperfusion model,and evaluate the nervus function coloboma of rats with the Bedersonl 5-grades evaluation method. Then use HE staining to observe the pathology of hippocampi. and using TUNEL method to detect apoptosis during different periods of change, Apply the method of West-Blot to test the protein expression of PDCD-5 and Caspase-3 in different time.Results:1.Neural function defect scale: the blank control group and control group of nerve function defect score of 0 points, cerebral ischemia reperfusion group(I/R group) and drug intervention group 0-4 points of neural function defect scale have, 0 and 4 point rats have been removed, from the same batch of rats randomly added, drug intervention group of nerve function defect score at the end of the(experiment) and less than those in cerebral ischemia reperfusion group, the difference was statistically significant(P < 0.05).2. HE staining results: in the blank control group and control group, visible SD rat hippocampus CA1 area neurons complete structure, clear boundary, not seen interstitial edema, no obvious infarct. Visible in the cerebral ischemia reperfusion group, the hippocampus CA1 area neurons cells necrosis, significantly decreased, and the lesion side neurons necrotic degeneration phenomenon increases with the increase over time. Drug intervention group compared with cerebral ischemia reperfusion group, is relatively more neurons, a clear structure, significantly reduce brain damage.3. TTC staining: the blank control group and control group did not see infarcts, and drug intervention group cerebral ischemia reperfusion group were clearly visible infarcts, and drug intervention group compared with cerebral ischemia reperfusion of cerebral infarction area percentage decreased, the difference was statistically significant(P < 0.05).4. TUNEL method to detect apoptosis: blank control group and control group with a small amount of cell apoptosis, in brain ischemia reperfusion group, the number of positive apoptosis cells in 6 h began to rise, peak when the 3 d, then gradually decline, but all the subgroups is still higher than control group and the blank control group, the difference has statistics meaning(P < 0.05). The apoptotic cells in the drug intervention group, the number of positive trends consistent with I/R group, also began to rise in 6 h, 3 d peak, then gradually decline, all subgroups with cerebral ischemia reperfusion group corresponding to the number of apoptotic cells subgroup is positive both decrease, the difference is statistically significant(P < 0.05).5. Western blot test results: the blank control group and control group in rat hippocampal CA1 area, can be detected and Caspase PDCD- 5-3 protein expression of PDCD- 5 in cerebral ischemia reperfusion group at 6 h and Caspase 3 began to rise, peak when the 3 d, then gradually decline, but all the subgroups is still higher than control group and the blank control group, the difference has statistics meaning(P < 0.05). PDCD- 5 in the drug intervention group and the expression of Caspase 3 trend is almost the same with I/R group, also began to rise in 6 h, 3 d peak, then gradually decline, all subgroups with cerebral ischemia reperfusion group corresponding subgroups compared PDCD- 5 and the expression of Caspase 3 decreased significantly, the difference is statistically significant(P < 0.05).Conclusion:1.The expression of PDCD-5 in hippocampus of cerebral ischemia/reperfusion rats is significantly increased.2. Edaravone can suppress the expression of PDCD-5 in hippocampus of cerebral ischemia/reperfusion rats, and can inhibit apoptosis,and may plays a role to protect hippocampal neurons of cerebral ischemia/ reperfusion rats. |
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