The Effect Of KCTD10 On The Invasion And Migration Of Gliomas

Objective: To detect the expression of KCTD10(potassium channel tetramerisation domain containing 10) in human gliomas, preliminary explorated the relationship between malignant degree of gliomas and KCTD10 and inquired the influence of KCTD10 on the migration and invasion of glioma cells at cellular levels.Methods: collected 15 cases of glioma samples, three glioma cell lines. using Western blot method to detect the expression of KCTD10 in clinical glioma tissues,Synthesised KCTD10 specific siRNA lentivirus, infected it into BT325 cells, 6 day after infection we detected the changes of KCTD10 at mRNA and protein levels. Scratch assay was used to detect the the migration of BT325 cells between the siRNA infection group and control group. Investigate the changes of migration and invasion of BT325 cells after silencing KCTD10 by Transwell assay and matrigel invasion assay. Using real-time quantitative PCR(Q-PCR) and Western Blot(WB) to explore the changes of invasion and migration related genes, such as MMP2, MMP9, VEGFA, HIF1 A and et al. Gelatin zymography was used to test the secretion of activated MMP2 in the infected cell culture medium.Results: Western blot assay showed that KCTD10 expression in gliomas was significantly higher than normal brain tissues, Scratch assay found lower migration in KCTD10 silenced cells(P<0.05), Transwell assay and matrigel invasion assay discovered a obviously decrease in migration and invasion after silencing KCTD10(P<0.05). Western Blot and Q-PCR detected apparent decline of MMP2, MMP9, VEGFA and HIF1A(P<0.05) in infected cells. Gelatin zymography indicated that the secretion of activity MMP2 was reduced.Conclusion: KCTD10 is highly expressed in gliomas, suggesting that it has a correlation with gliomas. Interferenced the expression of KCTD10 in glioma cells BT325 can significantly reduce its migration and invasion abilities and can also decline the mRNA and protein levels of MMP2, MMP9, HIF1 A and VEGFA, which suggested that the decrease of invasion and migration caused by disturbance of KCTD10 in BT325 cells may be realized by the regulating the signal pathway involved these genes.