The Irreversible Tipping Point Of Portal Hypertension In Glisson Pedicle Syndrome

Objective: To explore the irreversible tipping point of portal hypertension, and discuss the variation of biliary tract pressure, portal vein pressure and pathology by establishing SD rat model with biliary obstruction. Analyze the effect on the irreversible tipping point of portal hypertension in Glisson pedicle syndrome of TGF-β1/Smad3 signaling pathway.Methods: 1. To establish SD rat model of Glisson pedicle syndrome,rats were submitted to common bile duct ligation(CBDL) by laparotomy. 2.The common bile duct and portal vein changes were measured by B ultrasonic. Liver function was detected. 3.The portal vein pressure was measured by biological function experimental system. 4.We determined the hepatic fibrosis degree by HE、Masson staining, and observed dynamic changes and trends of heapticα-SMA and TGF-β1、Smad3 levels by Immunohistochemical image analysis in liver tissues of experimental rats.Results:1.SD rat models of Glisson pedicle syndrome were established successfully. 2.Biliary tract and portal vein pressures rose after common bile duct ligation. The portal vein pressures could reduce close to normal if relieving biliary obstruction early. Irreversible tipping point of portal vein pressure occurred if relieving biliary obstruction after modeling for more than 38 days. Pseudolobuli was observed meanwhile. 3.Immunohischemical results demonstrated TGF-β1 and Smad3 were positively correlated with the time of biliary obstruction.Conclusion: 1.Irreversible tipping point of portal vein pressure occurres after relieving biliary obstruction for more than 38 days when pseudolobuli was observed in SD rat models of Glisson pedicle syndrome. 2.TGF-β1 / Smad3 signal path is closely associated with portal hypertension of Glisson pedicle syndrome...