| ObjectiveTo investigate the efficacy of fenofibrate for hepatic steatosis in rats at early stage of severe scald.MethodsTwenty-seven male SD rats of clean grade were divided into sham group(n=9, sham injured on the back by immersing in 37℃ warm water for 15s), burn group(n=9, inflicted with 30% TBSA third-degree burn on the back by immersing in 98℃ hot water for 15s, and then intraperitoneally injected with Lactated Ringer’s solution 2 mL·kg-1·%TBSA-1 1h post burn), burn+fenofibrate group(n=9, treated as that in burn group at first, and then were treated with fenofibrate 80 mg·kg-1·d-1 24h post burn for 7 days while burn group were treated with normal saline instead). (1)3 rats of each group were sacrificed respectively at 4d,5d and 7d post burn, serum TC,TG,FFA,HDL and LDL content were determined with automatic biochemical analyzer, (2)After body mass weighing, liver were harvested for liver-body weight ratio record, observation of pathological changes by HE staining with light-microscope and count of each group’s rats at each degree by measurement of liver steatosis.The measurment data were processed with analysis of veriance of factorial design, and SNK test. And level information were assessed by Kruskal-Wallis analysis, and Nemenyi analysis.Results(1)At 4d post burn, the serum content of TC TG FFA in burn+fenofibrate group were decreased compared with burn group, with P value below 0.05, the HDL content were at same level, with P value above 0.05, no difference in LDL content among 3 groups, with P value above 0.05. At 6d post burn, the serum content of TC TG in burn+fenofibrate group weredecreased compared with burn group, with P value below 0.05, the HDL content were elevated compared with burn group, with P value below 0.05, no difference in serum content.of LDL and FFA among 3 groups, with P value above 0.05. At 8d post burn, the serum content of TC in burn+fenofibrate group were decreased compared with burn group, with P value below 0.05, the HDL content were at same level, with P value above 0.05, no difference in TG, LDL and FFA content among 3 groups, with P value above 0.05.(2)The livers in both burn+fenofibrate group and sham group feel soft and rubbery to the touch, and no oil staining on the liver slicing, while the burn group’s livers showed a faint yellow color and oil staining on the liver slicing obviously at 6d post bum. At 4 d post burn, the liver-body weight ratio of rats in 3 groups were at same level, with P value above 0.05.At both 6 and 8 d post burn, the liver-body weight ratio of rats in sham, burn, and bum+fenofibrate group were respectively 0.0416±0.0016,0.0533±0.0054,0.0370±0.0069 and 0.0423±0.0034, 0.0624±0.0005,0.044 4±0.0042. The liver-body weight ratio of rats in both burn+fenofibrate and sham group was lower than burn group, with P value below 0.05, but these two groups were at same level, with P value above 0.05.(3) Compared to the livers in sham group, typical hepatic steatosis was evidenced by a larger unstained area that consisted of small fat vacuoles in the livers of the burn group at 6d post burn. Fenofibrate treatment significantly reversed this change in the livers of the bum+fenofibrate group from 4d post burn on. Count of each group’s rats at each degree exhibit as follows, sham group:0 degree 9 rats, burn group:Ⅰ degree 1 rat, Ⅱ degree 1 rat, Ⅲ degree 7 rats, burn+fenofibrate group 0 degree 3 rats, Ⅰ degree 4 rats, Ⅱ degree 2 rats. Burn+fenofibrate group liver fatty degeneration was mild and reduced to normal gradually at 6d post burn Liver steatosis of rats in burn group was more severe than the other two groups, with P value below 0.05, the liver steatosis of rats in burn+fenofibrate group is more severe than sham group, with P value below 0.05.ConclusionFenofibrate can alleviate rats thermal injury-induced dyslipidemia and hepatic steatosis. |
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