The Clinical Value Of Hexokinase-Ⅱ Expression In Rectal Cancer

Objective:To detect the expression of Hexokinase-Ⅱ gene in rectal cancer and investigate the clinical value.Methods:Reverse transcription polymerase chain reaction, Western blot and immunohistochemistry were used to detect the expression of Hexokinase-Ⅱ gene in 100 cases of rectal cancer. The relationships between Hexokinase-Ⅱ gene expression and the clinical features such as tumor size, clinical stage, differentiation level, lymphatic metastasis were then studied.Results:Reverse transcription polymerase chain reaction showed that the RNA expression of Hexokinase-Ⅱ in rectal cancer tissue was significantly higher than that in normal tissue. Western blot indicated that the protein expression of Hexokinase-Ⅱ in rectal cancer tissue was significantly higher than that in normal tissue. Immunohistochemistry demonstrated that the rate of positive expression of Hexokinase-Ⅱ in rectal cancer cases was significantly higher than that in normal group (P<0.001). Tumor size, clinical stage and differentiation level were found to be related to the expression of Hexokinase-Ⅱ gene respectively (P=0.001, P=0.019, P 0.001), while lymphatic metastasis was not (P=0.910).Conclusions:The expression of Hexokinase-Ⅱ gene in rectal cancer tissue was significantly higher than that in normal tissue, and was related to tumor size, clinical stage and differentiation level. Thus, Hexokinase-Ⅱ gene could be a molecular marker for metastasis and invasion characteristics of rectal cancer.