| Background:With the continuous development of surgery and anesthesiology, more and more pregnant women and infants diseases can be treated by surgery, which makes an important developmental stage (after the birth of the last pregnancy 3 months to the second year) of the central nervous system inevitable exposure to under the stimulation of anesthetics. Development of the central nervous system in the mechanism of action of neurotransmitters and receptors are complicated, particularly in the predominantly synaptic plasticity development peak development period. For internal and external environmental factors appear to change, the nerve cell proliferation, migration, differentiation, the formation of synapses, the establishment of the loop to reshape and associative learning ability can be affected directly, and then followed by and lifetime of nerve, behavior ability and learning and memory function will also be affected. Isoflurane and sevoflurane are commonly inhalation anesthetics, of which neurotoxic effects on neural development has always been the focus of clinical anesthesia physician. However, the related mechanism of inhalation anesthetics to puberty brain function, there is still not clear. How to ensure the perfect anesthesia, at the same time to minimize neurotoxic effects on the development of the central nervous system, is the difficult problem that has plagued anesthesiologists, and also the focus research in the anesthesia community.Objective:To compare the effect of different time course of isoflurane on wnt3a and β-catenin gene expression and protein expression in the hippocampus of developing rats. To discuss the related mechanism of high concentrations of isoflurane processing of pubertal rat hippocampus nerve and the relationship with time.Methods:Thirty-two 7-day-old SD male rats were randomly divided into four groups:control group (A) inhalation of O2 instead of isoflurane,inhalation of 2% isoflurane 2 hours group (B), inhalation of 2% isoflurane 4 hours group (C) and inhalation of 2% isoflurane 6 hours group (D). Rats in every group were no hypoxia and CO2 accumulation occurred in the process.6 hours after treatment rats in every group were killed and hippocampus were removed immediately.Each group were divided into RT-PCR group(A1,B1,C1,D1) and western blot group(A2,B2,C2,D2) for determination of wnt3a and β-catenin gene expression and protein expression.Results:In the group of PCR of wnt3a, compared with control group, the level of wnt3a was increased markedly (P<0.05) only in group C1.While other groups have raised, there was no statistical significance; In the group of western bolt of wnt3a, compared with control group, the expression of wnt3a in groups of B2, C2, D2 were raised markedly (P<0.05), in which the group D2 rised significantly (P<0.01). In the group of PCR of β-catenin, compared with control group, the level of β-catenin was increased markedly (P<0.05) only in groups B1 and D1. While group C1 has raised, there was no statistical significance; In the group of western bolt of P-catenin, compared with control group, the expression of β-catenin in groups of B2, C2, D2 were raised markedly (P<0.05), in which the group D2 rised significantly (P<0.01).Conclusion:At different time of 2% isoflurane processing 7 day age of SD rat hippocampus wnt3a and β-catenin gene expression and protein expression, we found that the above protein expression in the hippocampus were raised as the extension of time, particularly six hours group. Therefore, it suggested that high concentrations of isoflurane processing of pubertal rat hippocampus nerve function was a positive correlation with time. This for us to explore isoflurane to puberty hippocampus nerve related role puts forward a new train of thought. Wnt3a and β-catenin expression increasing synchronously, which for us to study whether inhalation of high concentrations of isoflurane affected puberty hippocampal nerve related function by classic Wnt/β-catenin signaling pathways provides the direction of the organization. |
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