Study Of Autophagy-related Genes Atg3, Ambra1 Expressions In Chronic Rhinosinusitis Without Or With Asthma

ObjectiveAutophagy was an important mechanism of protection and defense in organisms. The purpose of the study was observed the expressions of autophagy-related genes Atg3 and Ambral in nasal mucosa, investigated the relevance between autophagy, chronic rhinosinusitis (CRS) without or with nasal polyps accompanied with asthma and discussed the probable pathogenesis mechanisms.MethodsCollection the nasal mucosa tissue of inferior turbinate in patients with nasal septum deviation (n=24)as control group,3 research groups included nasal mucous in ostiomeatal complex with chronic rhinosinusitis without nasal polyps (CRSsNP)(n=21), chronic rhinosinusitis with nasal polyps(CRSwNP) (n=22) and chronic rhinosinusitis with nasal polyps accompanied with asthma (CRSwNP accompanied with asthma) (n=18). The expressions of Ambral and Atg3 in each group were detected by Immunohistochemistry. Relevance of the autophagy-related genes among the groups was analyzed.Results(1) The expressions of autophagy-related genes Atg3 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were increased gradually (%2-31.080, P<0.001). There was statistically significant differences in expression used further pair-wise comparisons in the control group with CRSsNP group (P= 0.002), the control group and CRSwNP group (P< 0.001), control group and CRSwNP accompanied with asthma group (P< 0.001), CRSsNP group and CRSwNP accompanied with asthma group (P= 0.002), CRSwNP and CRSwNP accompanied with asthma group (P= 0.024). However, there was not any statistically significant difference between CRSsNP group and CRSwNP group (P= 0.304).(2) It was all gradually increased (x2=33.000, P＜0.001) in expressions of autophagy-related genes Ambral betwween control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group. There were statistically significant difference in expression used further pair-wise comparisons in the control group with CRSsNP group (P= 0.009), control group and CRSwNP group (P< 0.001), control group and CRSwNP accompanied with asthma group (P＜ 0.001), CRSsNP group and CRSwNP accompanied with asthma group (P< 0.001), CRSwNP group and CRSwNP accompanied with asthma group (P= 0.009). There was not any statistically significant difference between CRSsNP group and CRSwNP group (P= 0.205).(3) The expressions of Atg3 and Ambral were positively correlated in CRSsNP group (r=0.619,P=0.003), CRSwNP group (r=0.392, P= 0.022) and CRSwNP accompanied with asthma group (r=0.552,P= 0.033). However, there was no statistical correlation between Atg3 and Ambral in the control group (r=0.316,P-0.133).ConclusionsAutophagy is closely related with the CRS pathogenesis and may be involved in the occurrence of CRSwNP accompanied with asthma. Autophagy related genes Atg3 and Ambral may play a synergistic role in the development of CRS. Autophagy can be considered as a potential target for the diagnosis and therapeutic treatment in CRS.