| Objective:Explore the mechanism of breast cancer cells non-hormone dependence change hormone dependence, provide the theory basis for clinical non-hormone dependence breast cancer. Methods:(1) Transfection Exogenous SOCS3 gene into MDA-MB-231 cell by Lentivirus, Building SOCS3 over-expression breast cancer cell lines, RT-PCR and immunofluorescence test SOCS3 mRNA and protein expression. (2) Cloning experiments and MTT assay analyze the effects of exogenous SOCS3 gene in breast cancer cell growth.(3)Cell adnesion experiment and Boyden chamber to observe SOCS3 gene in breast cancer cell adnesion and mingration and invasion ability. (4) Western blot method to detect SOCS3 activity of STAT3, Akt signal molecules and the influence of the expression of cell cycle protein (cyclinD1, p21); SOCS3 rt-pcr and Western blot analysis and determination of p-MAPK, c fos, c-jun expression. (5) Treatment with tamoxifen carry SOCS3 gene of breast cancer cell lines, and did not carry SOCS3 gene of breast cancer cell lines are compared growth and apoptosis. Results:(1) Building SOCS3 overexpression breast cancer cell lines. (2) The grow SOCS3 over-expression breast cancer cell lines growth speed slower than normal cells, with significant difference. (3) SOCS3 expression of cell adhesion, migration and invasion ability weaker than normal cells. (4) SOCS3 expression in cells, STAT3 and Akt expression quantity increases, with significant difference. (5) At the same concentration of tamoxifen treatment, SOCS3 gene in breast cancer cell lines growth faster than not carry SOCS3 gene breast cancer cell lines growth speed is slow, accelerating cell apoptosis, with significant difference.Conclusion:(1) Succeed build on SOCS3 overexpression breast cancer cell lines. (2) SOCS3 of EGFR/C-erbB-2 negative regulation of signal pathways that EGFR/ C-erbB-2 in the expression of related genes strength downgraded. (3) The ER, PR expression level rised in SOCS3 overexpression breast cancer cell lines, is namely that the nonhormone dependence turn into hormone dependence and increases the chances of breast cancer patients to endocrine therapy. |
