| Hypochlorous acid(HClO) plays a crucial role in the innate immune system. However, deregulation of HClO levels is implicated with many pathophysiological consequences including cardiovascular diseases, rheumatoid arthritis, and cancer. Ruthenium complexes with three diimine ligands have attracted a great of interest in the field of luminescent probes and cellular chemosensors due to their desirable chemical and photophysical properties, such as good water solubility, high chemicalstablility and photostability, visible-light emission and absorption, large Stokes shifts, long lifetimes and low cytotoxicity. Ruthenium complexes with 1,10-phenanthroline ligands have higher quantum yield than the complexes with bipyridine ligands. In this thesis, A dinuclear ruthenium(Ⅱ) complex-based luminescent probe for HClO was successfully designed and synthesized by the modification of the phenanthroline ligand.Five ligands were designed and synthesized by conjugation of 1,10-phenanthroline and the recognizing moiety(aldehyde, carboxylic acid, malononitrile, 1-ethyl-2,3,3-trimethylindolenine, oxime) with linkers, respectively. One phenanthroline ligand was designed and synthesized by conjugation the 1,10-phenanthrolines with the recognizing moiety, an azo group. It was found that the ligand with an azo group had highly selectivity for hypochlorous acid and a dinuclear ruthenium(Ⅱ) complex Ruazo was synthesized by using the corresponding azo ligand.The luminescence responses of the ruthenium(Ⅱ) complex Ruazo for the detection of HClO were investigated and a method for the detection of HClO with the ruthenium(Ⅱ) complex was designed and developed. The non-emissive Ruazo can form highly luminescent triazole-ruthenium(Ⅱ) complex in presence of HClO and successfully imaged HClO in living cell and living mouse. |
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