Effect Of Amlodipine And Valsartan On Expression Of The NADPH Oxidase Subunits Gp91^phox And P22^phox In Human Coronary Artery Endothelial Cell

Objective To study the effect of amlodipine and valsartan on expression of Nicotinamide adenine dinucleotide phosphate（NADPH） oxidase subunits gp91 phox and p22 phox in human coronary artery endothelial cells（HCAEC）.Methods Experimental cells are 3¹0 generations of human coronary artery endothelial cells（HCAEC）for conventional subculture in vitro.The cells seeded onto a 10 cm cell culture dish at a cell density of 1 x 105/ml were allowed to reach 80% confluence.Then HCAECs were divided into control group and experimental group. The control group remained untreated,and the test groups were treated with different concentrations of amlodipine（1×10-5,1×10-6,1×10-7,1×10-8,1×10-9mol/L）and different concentrations of valsartan（1×10-4,1×10-5,1×10-6,1×10-7,1×10-8mol/L）,preincubated for 24 h.The NADPH oxidase subunits protein expression were assessed by Western blot analysis. The results of Western blot analysed with software Photoshop, and SPSS 13.0 statistical software was used to analysis the data,and multiple groups were compared using the One-Way Anova,the level of test is P = 0.05.Results Expressions of gp91 phox and p22 phox were significant in the experimental group and the control group. Amlodipine induced the expession of NADPH oxidase subunit p22^phox（P<0.05）. High concentrations of amlodipine significantly reduced expression of gp91 phox,while low concentration promoted the expression of gp91^phox（P<0.05）. p22 phox and gp91 phox were significantly inhibited by valsartan（P<0.05）, and the higher the concentration of valsartan,the greater the inhibition（P<0.05）.Conclusion Valsartan can inhibit oxidative stress and the higher the concentration,the greater the effect is.amlodipine suppresses part of NADPH oxidase subunit(gp91^phox) only at high concentrations,but it may increase oxidative stress in low concentrations.