The Characterization Of A Selfassembly Titanium/Drug-loading Nanoparticles Complex

Titanium and its alloys with good biocompatibility and rigid have been widely applied in the oral implant and the plastic surgery and they become the first choice of many bio materials. However the infection and inflammation caused by implant limited their application, so it is an urgent need to seek new titanium implants which can load and controlled release of therapeutic agents. In recent years, nanoparticle for sustained drug release has caused great attention and interest in the areas of both pharmaceutics and biomaterials. To this end, we synthesized a new non-ionic surfactant, Dop-Poloxamerl88, which is the modification of dopamine (Dop) in the both ends of Poloxamer 188.The new compound Dop-Poloxamerl88 had an ultraviolet absorption peak at about 285 nm, indicating that the successful modification of dopamine to Poloxamer188. Using Dop-Poloxamerl88 as a surfactant, PLGA as oil phase matrix and model drugs, drug-loaded nanoparticles with good characterization were prepared. The Dop-Poloxamerl88-PLGA nanoparticels had an average diameter of around 110 nm with polydispersity index (PDI) less than 0.1.Dopamine modified nanoparticles were immobilized on titanium surface through a simple dipping process. Then, the building of a new kind of drug loaded Ti/NP complex was completed. Water contact angle test, field-emission scanning electron microscope (Fe-SEM), fluorescence microscopic analysis and X-ray photoelectron spectroscopy (XPS) detection confirmed that dopamine modified nanoparticles were immobilized on titanium surface.Furthermore, After the examination of the Ti/NP complex, we found that Dop-P188-NPs almost has no cytotoxicity to the mice macrophage RAW264.7. Furthermore the Ti/NP complex could load Erythromycin (EM) and other antibacterial drugs, and showed good inhibitory effect to the growth of staphylococcus aureus.In conclusion, this study successfully constructed a novel drug-loaded nanoparticles which can be immobilized on titanium surface. The novel drug loaded Ti/NP complex has the potential to be used for site specific drug delivery and controlled release in the broad area of Ti based biomaterials.