The Study About SBP1 Gene Expression In Oral Tumors

Objective Selenium binding protein 1(SBP1) is a kind of special selenoprotein which is located on the chromosome lq21-22.SBP1 can inhibit tumorigenesis mainly through promoting tumor cell’s apoptosis.The study detected the expression levels of SBP1 in salivary gland tumor and clarified the relationship between SBP1 and oral tumor cells’(SCC3) occurrence, development, invasion and metastasis. Also the study investigated the potential of SBP1 as a tumor biomarker of oral tumors and evaluated the clinical prognosis.Methods 15 cases of benign salivary gland tumors and tumor adjacent tissues(pleomorphic adenomas) were collected and their m RNA were abstracted. The relative expression of SBP1 was quantified by SYBR Green real-time PCR. After transduction SBP1 overexpressd/silenced lentivirus into SCC3, m RNA expressions of related genes were measured. Migration ability was detected by cell scratch assay.Invasiveness was determined through MTT.Results 1.The lentivirus can effectively change the purpose gene expression, has the very high transfection efficiency. 2.QPCR showed that the expression of SBP1 in salivary gland tumor tissues was significantly lower than that in normal salivary tissues(P < 0.05). 3.Scratch test showed that the up-regulation of SBP1 expression could significantly inhibit the migration of SCC3.4.MTT showed down-regulation of SBP1 expression could promote the proliferation of SCC3.Conclusion 1.The expressions of SBP1 in salivary gland tumor and Peritumoral tissue were different, the results had statistical significance 2.SBP1 may inhibit tumor cell proliferation and migration ability. 3.The determination of SBP1 can be regard as an early diagnosis index of oral tumor.