The Apoptosis Effects Of NEMP On Rat Liver And Heart Tissue Cells

The human safety in Electromagnetic environment arouses raising concerns, and a lot of research work has been carried out to investigate the bioeffects caused by electromagnetic expose. It is found that as one type of electromagnetic pulse (EMP), the nuclear electromagniec pulse (NEMP) exposure also shows significant bioeffects. In the previous studies, it is found that the bioeffect of NEMP under a single exposure can be quantitatively described by an exposure-parameters and bioeffect equation, while using cell proliferation as the index. However, whether the the dose-bioeffect relation of living organs obey the same rule as the in vitro cells? Can the immediate effect recover after the single irradiation? In the case that no significant bioeffect is found in single exposure, will multiple exposures exhibit significant bioeffect? To know the answer for above questions, relavant studied have been carried out by using the liver and the heart of SD rats as the objects. The experimental results will give help for establish the security standard for the NEMP expose.In this paper, the damage on the liver and heart of the male SD rats that caused by NEMP under different irradiation conditions (electric field strength E=110 kV/m, 140 kV/m,297 kV/m and 873 kV/m; pulse number N=2,5 and 100), as well as the subsequent self-recovery behaviors were investigated, viaroutine blood test, biochemical examination (glutamic-pyruvic transaminase ALT, glutamic-oxalacetic transaminase AST), transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (Tunel, used for observing the apoptosis of tissue cells), and Enzyme linked immunosorbent assay (Elisa, used for detecting the content of two appptosis-related proteins Bax and Bcl-2).6h、1d、3d、7d、10d、15d after single exposure, above metioned indexes were detected to characterize the immediate bioeffect and self-recover property of the organs. The results show that the damage of the rat liver and heart were increased with the increase of exposure dose. No significant immediate bioeffect was found under a relatively low dose (E=110 kV/m, N=2). Significant immediate bioeffect was found under the exposure condition of low doae (E=110kV/m,140 kV/m, N=5, 100), and the detected indexes were revoveried within 1 day. Significant immediate bioeffect was also found under a relatively high doae (E=297 kV/m,873 kV/m, N= 100), and it seems not recovered within 10 days.According to the experimental result of immediate bioeffect and self-recovery, following expose parameters were chosen for subsequent accumulation experiment: E=110 kV/m, N=2 (no significant immediate bioeffect in the single irradiation), E=110 kV/m, N=5,100 (has immediate bioeffect in the single irradiation, and recovered within 1 day), E=297 kV/m, N=5,100 (has significant bioeffect in the single irradiation, do not recover in 1 day). The total exposure time is 7 d,14 d,21 d and 28 d, separately, with the frequency of 1 time pre day. The same experiment methods were used as above. The experimental results show that periodic multiple expose to NEMP will cause accumulated bioeffects, and the intensity of bioeffect of multiple expose is proportionate to the bioeffect of the single expose, which can be simplely described by a linear equation in this study.