The Co-influence Of S100A16 And 11β-Hydroxysteriod Dehydrogenase On Glucolipid Metabolism

S100A16 protein is a widely expressed calcium binding protein in various body organizations. It belongs to the S100 protein family. It has the function of regulation and extracellular calcium ion homeostasis. In the body it plays an extensive and important biological functions.Current research suggests that S100A16 affect the development of a variety of tumors, adipose differentiation and the lipid accumulation. In our previous study we found that in mouse adipose cells (3T3-L1) the expression of S100A16 down regulated 60%when disturbances expression of 11β-hydroxysteroid dehydrogenase 1. We hypothesize that S100A16 jointly with 11β-HSD1 may play an important biological function in lipid metabolism, but the exact mechanism is unclear.By using transgenic mice models, this study is designed to give an examination of the role of S100A16 and 11β-HSD1 in energy metabolism related to diet-induced obesity. Aged 5 weeks mouse were randomly divided into four group, which is C57BL/6 mouse with normal diet (n=10), S100A16Tg-/+mouse with normal diet (n=10), C57BL/6 mouse with high-fat diet (n=10) and S100A16Tg-/+ mouse with high-fat diet (n=10), which are providing different diets for 17 weeks to establish a diet induced obesity (DIO) mice model. At 15 and 21 weeks, mouse were processed intraperitoneal glucose tolerance test (IPGTT) and insulin tolerance test (ITT). When mouse were executed after 17 weeks, weighted subcutaneous fat weight. Then we used HE staining method (hematoxylin and eosin staining, HE) to observe the liver and fat pathological changes, radiation immunity analysis to check blood serum biochemical indicator and Western blotting to observe the expression of S100A16, 11β-HSD1 and transcription factors in liver and adipose tissue. S100A16 and 11β-HSD1 can promote adipose accumulation.In 3T3-L1 cell model, we found that when S100A16 and 11β-HSD1 are both overexpressed, there have a significant increasing in differentiation of 3T3-L1 cell and promoting adipogenesis.These can be manifested by the markedly elevated cellular lipid content and the expression of adipocyte marker genes.