Effect Of Human Ovarian Cancer Cell On Human Umbilicail Cord Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are a kind of adult stem cells that have the potential of self-renew and multi-differentiation, can differentiate into mesenchymal tissue, as well as to be induced into a variety of tissue cells. Human umbilical cord mesenchymal stem cells (HUMSCs) derived from the newborn’s umbilical cord Huatong plastic, as they are not limited to ethical constraints, and with low immunogenicity as well as have high proliferation and so on, rising them to be the seed cells of cell therapy. Recently, HUMSCs become a hot research area in regenerative medicine, cell transplantation and tumor therapy. In this research, we used the co-culture HUMSCs with ovarian cancer (HO8910) in transwell, to explore the impact of tumor microenvironment to the biological activity of HUMSCs.However, the mechanism of HUMSCs in treating tumor still need more research.HUMSCs were obtained by the "round coverslip" method. Flow cytometry was used to test HUMSC surface markers, co-culture HUMSC with ovarian cancer (HO8910) used Transwell, MTT assay was used on stem cell proliferation and migration, the apoptosis of HUMSC determined by Western Blot.Flow cytometry analysis showed high expression of CD 105, CD90, CD73, CD29, but low expression of CD45, CD34 and HLA-DR in HUMSCs. MTT assayed the proliferation of co-cultured HUMSC, its proliferation, migration and apoptosis hadn’t been effected after culture with supernate of HO8910 compared with the control group, while using indirect co-culture transwell resulted in promoting the proliferation and migration of HUMSCs.This study confirmed that indirect co-culture HO8910 with HUMSC will promote the proliferation and migration of HUMSC, which provides theoretical basic for further study on HUMSCs in cell therapy.