Effect Of SiRNA C-Met On The Biological Characteristics Of Esophageal Squamous Cell Carcinoma Cells

Objective: To investigate the effects of siRNA c-Met on the biological characteristics of esophageal squamous cell carcinoma cell line CE81T-4. Methods: In this study, we used siRNA technique to stably transfected CE81T-4 which c-Met siRNA is packaged by slow virus of fluorescence. c-Met siRNA for transfection of CE81T-4 was established as the experimental group. Transfection of empty slow virus as negative control. Non transfected esophageal squamous cell carcinoma CE81T-4 as blank control. The transfection effect was observed under the inverted fluorescence microscope. The expression of mRNA c-Met in three groups of cells was detected by qRT-PCR. MTT, scratch test, Transwell and flow cytometry were used to detect the proliferation, migration, invasion, cycle and apoptosis of the three groups of cells. Results: After transfection with siRNA c-Met, the expression of green fluorescence was observed under fluorescence inverted microscope. The expression level of mRNA c-Met in the experimental group was significantly lower than that in the negative control group and blank control group(P﹤0.05, P ﹤ 0.05). The cell proliferation ability of the experimental group was significantly lower than that of the negative control group and blank control group, the difference was statistically significant( P ﹤ 0.05). Transwell in vitro invasion experiment showed that the number of invasion and migration of the experimental group( 135±11.79) was significantly lower than that of the blank control group(186±14.98)and the negative control group(178±12.53)(P﹤0.05). The apoptosis rate of the experimental group(17.87±1.60)% was higher than that of the negative control group(4.93±2.49)% and blank control group(4.37±1.60)%,the difference was statistically significant( P﹤ 0.05). The G0/G1 phase cells of the experimental group( 48.57±4.9 1) % were increased compared with the negative control group(38.13±3.23)% and blank control group(37.07±2.32)%(P﹤0.05), the difference was statistically significant. Conclusion: siRNA c-Met can increase the apoptosis rate of esophageal squamous cell carcinoma cells, the cycle is blocked in the G0/G1 phase, which can reduce the proliferation, migration and invasion ability.