A Preliminary Study Of Detecting Meningioma Stem Cells In Salvaged Autologous Blood

ObjectiveTo investigate the amount of meningioma stem cells in the salvaged blood which had been mixed with anticoagulant, filtered, centrifuged, washed and purified through the autologous blood transfusion device. In this study, Flow cytometry was used to detect the amount of meningioma stem cells. This study can supply experimental evidence for the application of intraoperative autologous blood transfusion in meningiomas and evaluate the safety of this technique.MethodsFrom June 2014 to October 2015, 89 patients with meningiomas who have the surgical treatment in the fifth affiliated hospital of Zhengzhou Universitywere selected. 19 patients of them were exclude because of the rejection of salvaged from their family, intracranial infection, or ASA>Ⅲ and so on, and finally there were 70 patients available. Autologous blood transfusion was used throughout the surgery.The procedure of salvaging blood, washing and transfusion was strictly followed during the operation. Take a little of the blood around the meningioma(B1), blood inthe salvaged container(B2) and blood after washing and centrifuge(B3) and make blood smear separately. Observe the shape of the cells through the light microscopy.B1, B2 and B3 blood samples were obtained and detected through flow cytometer and direct immunofluorescence method, and observe B1, B2 and B3 separately the expression amount of CD133 and CD166, and save the results as dot diagram.The meningioma tissue was embedded in paraffin, dyed through hematoxylin-eosin staining(HE),and examined through immunohistochemical method.ResultsThe average years of the 70 patients was 52.87±17.29, 37 patients were male(52.86%), and the other 33 patients were female(47.14%). 22 patients were ASAⅡ(31.43%), and the other 48 patients were ASA Ⅲ(68.57%). The total amount of the salvaged blood was 25176 ml, and the average amount for every patient was359.66±169.72 ml. In B1 blood many tissue fragments, destroyed cellular matrixs and so on were observed under the light microscope, but meningioma stem cells were not found. Less tissue fragments, destroyed cellular matrixs and so on were observed under the light microscope, but meningioma stem cells were also not found in B2 blood. There were only red blood cells can be observed in B3 blood. All patients were diagnosed with meningiomas by pathology, and there were several pathological characteristics can be observed under the light microscope through the immunohistochemical method. The results of flow cytometer showed that the expression rate of positive CD133 was 0.54% in B1 blood, and the expression rate of positive CD166 was 3.46%, but the expression rate of both the two was zero. In B2 blood the expression rate of positive CD133 was 0.43% in B2 blood, and the expression rate of positive CD166 was 3.58%, but the expression rate of both the two was also zero. In B3 blood, the expression rate of positive CD133 was 0.39%, and the expression rate of positive CD166 was 2.75%, but the expression rate of both the two was also 0.ConclusionThe meningioma stem cells have not been found in the salvaged blood which had been mixed with anticoagulant, filtered, centrifuged, washed and purified, and the safety of the application in meningiomas can be demonstrated. The intraoperative autologous blood transfusion in operation can effectively reduce the amount of allogenic blood transfused and relieve the pressure of the short of blood.It should be applied more widespread.