| Peripheral nerve defect is the clinical common complications, most of the patients with part of nerve function loss or disabled for life. With the deepening of the research, tissue-engineered nerve are used to replace autogous on repairing peripheral nerve, and have acquired some effect. This paper used tissue-engineered nerve which made by allograft bone MSCs (BMSCs) and acellular nerve allografts (ANA), to transplant and repair canine sciatic nerve 3 cm defects. After the surgery, testosterone propionate (TP) was injected into the gastrocnemius. Then, autograft group、ANA-BMSCs group、ANA group and nongrafted group were set as control. And, the repair outcomes were evaluated by a combination of electrophysiological assessment, FluoroGold retrograde tracing, and histological investigation to regenerated tissue and reinnervated target musle at 5 months after nerve grafting. Then results were got as follows:1. The sensorimotor function recovery time of TP-joint group and autograft group were 3.5M, which was earlier than ANA-BMSCs group (4M). Three groups all could stand with hind legs and had slight claudication. TP-joint group could jump off the ground while others were not observed this situlation. The recover effect of ANA group was poorer, and nongrafted group was poorest.2. The recovery percentage of conduction velocity in TP-group (33.19±3.59%) was no significant difference with that of autograft group (39.79±12.03%) (P>0.05), the recovery percentage of amplitude in TP-joint group (A:48.84±3.28%, B:48.06±6.17%) was significantly higher than that of autograft group (A:45.55±3.50%, B:43.25±3.42%) and ANA-BMSC group (A:28.22±1.73%,B:44.97±1.37%) (P<0.05).3. There was no significant difference of gastrocnemius muscle wet weight ratio among these three groups (TP-joint group:80.62±0.96%, autologous groups:76.64±3.93%, engineering group:72.02±10.22%). The recovery percentage of muscle fiber area in TP-joint group (48.78±8.18%) had no significant difference with autograft group (43.78±11.45%) (P>0.05), and had significant difference with ANA-BMSC group (35.88±8.10%) (P<0.05).4. Anatomical observation showed the regenerated nerve of autogroup and TP-joint group was similar to the normal one, and was superior to that of other groups.5. FG retrograde tracer showed that the density of dorsal root sensory fluorescent neurons in TP-joint group was similar with ANA-BMSC group and slightly lower than that of autograft group, and the density of spinal motor fluorescent neurons in TP-joint group was slightly higher than that of ANA-BMSC group and autograft group.6. The histological observation showed that the regenerative density of myelinated nerve fiber in TP-joint group slightly higher than that of ANA-BMSC group and autograft group. The recovery percentage of myelinated nerve fiber diameter in TP-joint group (47.84± 10.38%) was no significant difference with autograft group (43.58±12.60%), but significantly higher than that of engineering group (33.16±6.72%) (P<0.01). The recovery percentage of the thickness of myelin sheath in TP-joint group (63.48±9.85%) was significantly higher than that of autograft group (53.76±12.01%) (P<.01).Comprehensive evaluation showed that testosterone propionate could significantly promote BMSCs-ANA to repair canine sciatic nerve defect, TP-joint group and BMSCs-ANA group both could repair 3 cm long nerve defect good, but the former had a better result, it could reach or exceed the effect of autologous group. |
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