Antibacterial Effects Of Single Or Compound Preparations Of Ten Commonly-used Materia Medica To Acinetobacter Baumannii In Vitro

In recent years, multiresistant acinetobacter baumannii has become one of the five major drug-resistant bacteria, even the first in various hospitals. Ab is prone to be drug-resistant, which often leads to clinical failure. Since there are few effective antibacterial drugs, we attempt to adopt traditional Chinese herbs to directly work on or assist the anti-bacterial treatment. China owns abundant resources of traditional Chinese medicine. In this experiment, ten materia medica with good antibacterial effects such as schisandra chinensis, scutellaria baicalensis, coptis chinensis, fructus forsythiae, spatholobus sinensis, pericarpium granati, lysimachia christinae hance, herbaandrographitis, allium sativum and galla chinensis were successively administered to act on acinetobacter baumannii(Ab) with a unified approach. Through the antibacterial experiment in vitro, inhibition zones of different single drugs and compound preparations were observed. The effects to Ab’s minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) were detected simultaneously. Besides, the antibacterial effects of joint use of garlic were tested. The experiment was finished through the following steps: clinically collecting, separating, culturing, purifying and identifying Ab samples; extracting the decoction(single and compound preparations); determining bacterial inhibition zone(Oxford Cup method); determining of MIC(96-Well Plate method); determining MBC.As for one single drug, the antibacterial effects tend to be: garlic> schisandra = spatholobus sinensis =pericarpium granati > galla chinensis > scutellaria baicalensis, coptis chinensis france, weeping forsythia, dichondra repens forst, andrographis paniculata. Antibacterial effects of the combination of garlic and Chinese herbs to MDRAb in vitro: the combination of spatholobus and garlic tends to be the best, which is still not as good as pure garlic juice, since the garlic mixture adversely affects the pure garlic juice. Antibacterial effects of five compound prepartions to MDRAb in vitro: insecticide and detoxification group, composed of spatholobus and garlic, is the most effective. The second best is the other groups. While pure garlic juice is still the best. Antibacterial effects of all compound preparations to MDRAb in vitro: garlic > insecticide and detoxification group > mixture group> garlic + pericarpium granati≈garlic + schisandra > garlic + pericarpium granati + schisandra + galla chinensis > galla chinensis = spatholobus sinensis = pericarpium granati. MIC results indicated that garlic group, insecticide and detoxification group had significant antibacterial effects to Ab, which was proportional to the concentration within certain limits. 3. MBC results showed that insecticide and detoxification group and garlic had significant antibacterial effects to Ab.The following findings can be concluded from the experiment: garlic juice, insecticide and detoxification group, mixture group, garlic + pericarpium granati, garlic + Schisandra have significant antibacterial effects, which can markedly inhibit the propagation of Ab in vitro. Instead of improving the antibacterial effects, garlic mixture affected the effect of sole garlic, it is thus not recommended to combine garlic with these drugs in vitro application. The effect of preparation is also not as good as sole garlic, while it is the second best. From this experiment, we can find that after recomposition, two groups of compound preparations with weak antibacterial effects become stronger instead. Therefore, these issues need further study, We hope we can expand the screening scope of materia medica to study their antibacterial effects to Ab.