The Effect Of Myricetin On The Proliferation Ability And The Cytokine Secretion Of Human Periodontal Ligament Cell Population

Objective The aim of this study was to explore the potential beneficial effect of effective components extracted from natural plants on treating periodontal disease by observing the anti-inflammatory activity of Myricetin on human periodontal ligament cell population(PDLP) stimulated by lipopolysaccharides(LPS) from escherichia coli, viewing to provide the theoretical basis for the rational medicine in the treatment of periodontal disease.Methods 1. PDLP were primarily cultured with tissue culture method. Then the growth conditions of PDLP were observed under inverted phase contrast microscope. Moreover, immunohistochemical staining methods were used for identify the sources of PDLP and the cell growth curve was determined by MTS assay. Besides, the mineralization induction was conducted and mineralized nodules were observed under inverted phase contrast microscope after alizarin red staining. 2. The influences of Myricetin in different concentration on the proliferation and differentiation of PDLP were investigated by MTS assay and ALP Kit. 3. The influence of Myricetin on the proliferation of PDLP stimulated by LPS from escherichia coli was conducted by MTS assay. Besides, IL-1β and TNF-α secretion of PDLP stimulated by LPS from escherichia coli was detected using enzyme-linked immuno sorbent assay(ELISA).Results 1. PDLP in normal and good condition were seperated from tissue fragments in 5-12 days. The growth curve model of PDLP looked like a inverted “S”. The vimentin staining was in positive and the keratin was in negative by immunohistochemical analysis. The osteogenic induction of PDLP showed that: mineralization nodule was formed and alizarin red staining was in positive. 2. The MTS assay results showed that Myricetin(10 μM/L, 20 μM/L) could increase the proliferation of PDLP,and there were significant differences when compared with control group(P<0.05). Besides,the ALP Kit results showed that Myricetin(5 μM/L, 10 μM/L, 20 μM/L) could increase the ALP activity of PDLP(P<0.05)and the rising tendency was dose dependent. 3. The MTS assay results showed that Myricetin in 20 μM/L could reduce the growth inhibition of PDLP stimulated by LPS from escherichia coli. Moreover, Myricetin in 20 μM/L could inhibit cytokine secretion of PDLP stimulated by LPS from escherichia coli both in IL-1β and TNF-α.Conclusion 1. Myricetin can increase the proliferation ability of PDLP at certain concentration. 2. Myricetin can increase the osteogenesis ability of PDLP at certain concentration. 3. Myricetin can protect PDLP against growth inhibition caused by LPS from escherichia coli at certain concentration. 4. Myricetin can inhibit cytokine secretion of PDLP stimulated by LPS from escherichia coli both in IL-1β and TNF-α at certain concentration.