Effect Of Glutamine On P38MAPK And IL-17 Expression In PBMC Of Patients With Chronic Obstructive Pulmonary Disease

Background Chronic obstructive pulmonary disease(COPD) is a kind chronic inflammatory lung disease which limited in progressive and not completely reversible airflow.The morbidity and mortality rate is very high in worldwide thus become a serious threat to the quality of human life. The morbidity and mortality of COPD rise year by year, which is expected ranked the 3rd cause of death in 2020. According to the survey of the World Bank and the World Health Organization study predicts that COPD will be the fifth world’s economic burden of disease.What’s more, COPD become the first causes of death in ruraland fourth in city in China, which bring huge social and economic burden to the world.It is an important public health problem all over the world.Patients with COPD have close relationship with respiratory inflammation which cause by harmful gases and particles, It not only affects the lungs but also can affect multiple extrapulmonary systems with the progress of the disease,including the cardiovascular system diseases,osteoporosis,anxiety,depression.The exacerbations of COPD and complications affecting the prognosis of the disease, which influence the patient’s quality of life seriously and bring huge mental burden to the patients. The pathogenesis of COPD is not entirely clear at present, numerous studies have shown that inflammatory cell activation and transcription play an important role in occurrence and development process of pulmonary inflammation. So the domestic and international scholars are focus on how to block or inhibit the activation of inflammatory cells and proinflammatory factor link effectively, thus achieve the purpose of prevention and treatment of COPD.p38 MAPK pathway is mainly mediated extracellular signaling to the nucleus, which can be activated by variety of stress, inflammatory factors, which affects the cell transcription, protein synthesis and cell surface receptor expression and it also has closely related with cell apoptosis, immune adjustment and regulation of the inflammatory response. It is the key to trigger inflammation thus start the inflammatory cells release a large number of cell factor.Glutamine(Gln) is a kind of essential amino acids of human body under the condition of stress state, which has lots of function,such as antioxidation and immune regulation.Howerer the study of anti-inflammatory effect of COPD by Gln is very little.The mechanism of anti-inflammatory of the Gln can inhibit the activation of p38 MAPK pathway and decrease cytokine expression,it is limited to animal experiments.Especially, it can inhibit p38 MAPK pathway and decrease the levels of IL-17 so as to achieve the anti-inflammatory effect of COPD patients has not been reported at home and abroad by now.Objective To determine the effects of glutamine on the p38 Mitogen-Activated Protein Kinases(p38MAPK) activity and IL-17 expression in peripheral blood mononuclear cells(PBMC) of patients with chronic obstructive pulmonary disease(COPD).Results The levels of p38 MAPK and IL-17 m RNA gene expression in AECOPD blank control group and SCOPD blank control group were higher than those in the normal group(P<0.01 and P<0.05, respectively). Moreover, there was a significantly increase both p38 MAPK and IL-17 m RNA gene expression in AECOPD blank control group compared with SCOPD blank control group(P<0.05); The levels of p38 MAPK and IL-17 m RNA gene expression in AECOPD Gln group were higher than those in the normal group(P<0.05), however there was no significant difference between SCOPD Gln group and the normal group; The levels of p38 MAPK and IL-17 m RNA gene expression in AECOPD Gln group and SCOPD Gln group were significantly lower than those in blank control group(P<0.01 and P<0.05, respectively).Conclusion Gln could inhibit p38 MAPK cell signaling pathway and reduce the expression of IL-17 in PBMC of patients with COPD.