Study On The Role And Mechanism Of IL-1β Promote Proliferation And Migration Of Lung Squamous Carcinoma SK-MES-1 Cell

Background Lung cancer is one of the most common malignant tumor of the clinical. At present, the mortality rate has is the first worldwide malignancy with gradually increased of morbidity. Lung squamous carcinoma is the important histological types of lung cancer, but the research results about it are very rare. A growing number of studies have confirmed that the development of tumor is associated with chronic inflammation. Inflammation is not only can increase the probability of cancer also can promote the development and evolution of the tumor. IL-1β is the center of the inflammatory reaction, there has been a lot of reports confirmed that IL-1β accelerate the development of tumor. miRNAs are non-coding small RNA involved in the negative regulation in the body,through influence the expression of encoding genes to widely participate in various life activities. Studies reported that miR-223 expression reduced in rats and human lung after smog exposuring. Nevertheless.the relationship of IL-1β and progression of lung squamous carcinoma, as well as are there miR-223 participate in the regulation are not clear.Hence, this research through the simulation of inflammatory environment in vitro to explore the role and mechanism of IL-1β in proliferation and migration of lung squamous lung squamous carcinoma, offers a new breakthrough point for early prevention and treatment of tumor.Objective (1) To explore the influence of IL-1β in lung squamous cell cancer proliferation and migration.(2)To revel the concrete mechanism of IL-1β promote lung squamous cell cancer proliferation and migration.Method (1) Through cloning experiments to observe the effects of SK-MES-1 cell proliferation by different concentrations IL-1β.(2) Through scratch test and Transwell experimental to observe the effects of migration ability of SK-MES-1 by IL-1β.(3) By fluorescence quantitative PCR to detect miR-223 expression.(4) To detect lung squamous carcinoma proliferation by CCK8 and migration by Transwell after transient transfection of miR-223 mimics, mimics-NC、inbihitor、 inbihitor-NC to SK-MES-1 cell.(5) To detect expression of COX2 after transient transfection of miR-223 and detect expression of COX2 after IL-1β treatment SK-MES-1cell.(6) To detect P65 protein nuclear transfer after IL-1β and PDTC treatment by cell immunofluorescence method.(7) To detect miR-223 expression by fluorescence quantitative PCR after PDTC blocked NF-κB signal path.Result (1) Inflammatory cytokines IL-1β can promoter lung squamous cancer cells proliferation and clone formation in concentration-dependent manner. This effect is the most significant at lng/ml,but this action was no longer increased proportionally When the concentration of IL-1β above lng/ml.(2) Transwell experimental and scratch test have shown IL-1β can boost lung squamous cancer cells migration ability.(3) IL-1β reduce the expression of miR-223 in concentration-dependent and time-dependent manner.(4) The up-regulation miR-223 by transient transfection inhibit proliferation and migration of lung squamous cancer cells, prompting that miR-223 may is tumor suppressor genes in lung squamous cell cancer.(5)The expression of COX2 is negative rugulated by miR-223. Besides, IL-1β can enhance COX2 expression.(6) IL-1β activate NK-κB and promote P65 protein transposition into the nucleus and it is blocked by PDTC.(4) IL-1β reduce the expression of miR-223 expression depend on NF-κB signaling pathways Conclusion IL-1β can activate NF-κB signaling pathways to reduce miR-223 expression,making its target genes expression of COX2 increase to promote proliferation and migration of lung squamous carcinoma. IL-1β/NF-κB/miR-223/COX2 control loop provide a new angle of view to explore the relationship between inflammation and lung squamous carcinoma and its molecular mechanism, may also provide new ideas to tumor research and treatment.