| Aim:To assess the effect of long-term oral inoculation with Porphyromonas gingivalis (Pg) on lipid metabolism disorders in apolipoprotein E-knocked out (ApoE-/-) mice and to explain the possible mechanism on inflammation and lipid metabolism disorders.Methods:Sixteen Specified Pathogen Free male ApoE-/- mice were randomly divided into 2 groups. ApoE-/--Pg group was inoculated orally with 109/ml Pg 33277 for 9 weeks, while ApoE-/--Ct group with PBS. Blood biochemistry method was used to test the level of TC, TG, HDL, LDL in the serum. Elisa method was used to test the level of specific IgG antibody of Pg, IL-6, MCP-1, ox-LDL in the serum. Red oil "O" stain was used to observe the plaque area and in the aorta root. HE was used to observe the histopathological changes in liver. Real Time Ouantitative-PCR was used to monitor the CD36, ABCA1, ABCG1 expression in the aorta.Results:1. plaque area in the aorta and Vacuolar degeneration of liverComparing to the ApoE-/--Ct group, in ApoE-/--Pg group the plaque area in the aorta was higher (P<0.05), and the vacuolar degeneration of liver was more serious.2. Inflammatory cytokines and Blood lipid levelComparing to the ApoE-/--Ct group, in ApoE-/--Pg group the level of TC, TG, LDL, ox-LDL was significantly increased and the level of HDL was significantly reduced(P＜0.01), the level of IL-6, MCP-1 was significantly increased(P< 0.001).3. Transcription of CD36, ABCA1, ABCGl in the aortaComparing to the ApoE-/--Ct group, in ApoE-/--Pg group the transcription of CD36 was significantly increased(P<0.01), while the transcription of ABCA1 and ABCGl had no significant difference(P>0.05).Conclusion:Oral inoculation with Pg in ApoE-/- mice could deteriorate inflammation in the body, aggravate the lipid metablosim disorders, aggravate the vacuolar degeneration of liver, accelerate the formation of plaque in the aorta and promote the cholesterol influx in the aorta, which was mediated by CD36 and may be one of the mechanisms of atherosclerosis. |
PDF Full Text Request