The Study On Preparation Process And Quality Standard Of Centralizer Liver Granules

Objective: Study the centralizer liver granule preparation process、quality standard and to establish a bitter butyl tea quality standards.Methods:(1)Using single factor experiment with orthogonal experiment method, astragalus glycosides content and the yield of extract from comprehensive score for evaluation index optimizing technological parameters of huangqi herbs alone water extraction;(2)Using single factor experiment with orthogonal experiment method, with the content of chlorogenic acid and the yield of extract from comprehensive score for evaluation index optimization wormwood and malaytea scurfpea fruit, bitter butyl tea herbs such as water extraction process parameters;(3)Using single factor experiment with orthogonal experiment method, in order to rhubarb phenol content and the yield of extract from comprehensive score for evaluation index optimizing rhubarb, cassia seed, hawthorn and alisma medicinal alcohol extraction process parameters, such as;(4) The best molding process, with 70% ethanol as moistening agent, to extract powder 25% of notoginseng powder, 38% of the dextrin granulating.;(5)Using thin layer chromatography Identify the centralizer liver granule of huangqi, notoginseng, wormwood, bitter butyl tea, malaytea scurfpea fruit, rhubarb and other chinese herbal medicine composition using high performance liquid chromatography method for determining the content of astragalus armour glycosides in preparations, the content of chlorogenic acid and rhubarb phenol content and methodology of the study on developing quality standards;(6)Establish the quality standards of bitter butyl tea.Result:(1)Centering the liver particles alone for 8 times the best water extraction process of water reflux extracting 3 times in a row, each 90 min;(2) The best water extraction process for 10 times the amount of water reflux extraction 2 times in a row, each 60 min;(3) The best alcohol extraction technology of 10 times the amount of 80% ethanol reflux extraction 3 times in a row, every time 30 min(4) The best molding process, with 88% ethanol as moistening agent granulating;(5) Established in preparations and radix astragali, notoginseng, wormwood, bitter butyl tea, malaytea scurfpea fruit, TLC identification method of rhubarb, by high performance liquid chromatography determination of astragalus in righting adjustable liver particles, the content of chlorogenic acid, methyl glucoside content rhubarb phenol content, chromatographic conditions are: mobile phase of acetonitrile and water respectively(all 9), 0.05% acetonitrile- phosphate(10:9 0), methanol- 0.1% phosphoric acid(85:15), detector for evaporative light-scattering detector, uv detector detection wavelength of 327 nm and 254 nm, respectively column temperature 30 ℃, the flow rate of 1.0 mL/min;(6)The bitter butyl tea character, microscopic identification were described, to make the bitter butyl tea TLC identification method of chlorogenic acid in; item in the check book bitter butyl tea water may not be over 13%, the total ash content may not be over 13%, acid insoluble ash content may not be over 5%. Determine the content of chlorogenic acid in bitter butyl tea determination method, and the temporary order according to the results of the determination of contains chlorogenic acid shall not be less than 0.60%.Conclusion: Righting the liver granule production process reasonable and feasible; formulating the quality standard is simple, rapid and accurate, and can effectively control the quality of the preparation.