Study On Effect Of Polarity Protein Par3 On Proliferation, Migration And Invasion In Cervical Cancer Cells

Objective: The aim of this study was to investigate the expression of Par3 protein in cervical carcinoma and whether Par3 regulate cervical carcinoma growth and metastasis. Methods: Immunohistochemistry were used to analyze the expression of Par3 protein in samples from 89 cervical squamous cell carcinoma. qRT-PCR were used to detect the basic expression of gene PARD3 in cervical carcinoma SiHa cells infected with HPV and C33 A cells infected without HPV. Transfected cervical carcinoma SiHa cells by the technology of gene overexpression and RNA interference. qRT-PCR and Western Blot were used to detect PARD3 mRNA and protein levels. Transwell Chamber in vitro testing assessed their effects on invasion and migration of SiHa cells. The cell-cycle and cell apoptosis were examined by flow cytometry. Results: The differences of expression of PARD3 had statistical significance between cervical carcinoma SiHa and C33 A cells. Expression of Par3 in normal cervical epithelia and weak expression in CSCC tissue in immunohistochemistry; qRT-PCR and Western blot showed that the expression of mRNA and protein of PARD3 in SiHa cells transfected by over-expressed plasmid was significantly increased and decreased in cells with PARD3 siRNA. Lost of Par3 induces MMP-9 expression and epithelial to mesenchymal transition(EMT) related genes(N-cadherin, E-cadherin and β-Catenin) expression changed in SiHa cells. Transwell Chamber in vitro experiments showed that in over-expressed group cell invasion and migration was significantly lower than the control group(P<0.05), and in down-regulated group cell invasion, migration was significantly higher than the control group(P<0.05), compared with the blank group, over-expressed PARD3 caused SiHa cell cycle arrest at the S phase, apoptosis rate decreased significantly in the experimental group(P<0.01). However, down-regulated PARD3 made cells arrest at the G0/G1 phase, rate of apoptosis significantly increased in the experimental group(P<0.01) in the flow cytometry results. Conclusions: The reduced Par3 expression in cervical cancer promotes the capacities for proliferation, migration and invasion.