The Level And Its Clinical Significance Of IFN-γ、IL-4、 TGF-β And IL-17 In Serum Of Patients With Chronic Lymphocytic Leukemia

Objective:This study was to investigate the clinical significance of interferon-γ(IFN-γ), interleukin-4(IL-4), factor-β(TGF-β)and interleukin-17(IL-17) level in serum of patients with chronic lymphocytic leukemia(CLL) and explore their potential role in the prognosis and clinical significance.Methods: The study enrolled 20 healthy individuals as controls and 60 CLL patients as a patient group,including 16 patients in the stage of BinetA,25 in the stage of BinetB,and19 in the stage of Binet C;32 received treatment and follow-up. ELISA was used to measure the level of transformation growth IFN-γ, IL-4, TGF-β,IL-17 in the serum of each group. Application software SPSS17.0 statistical was used for statistical analysia,quantitative data was sigified by the mean±standard.T test was used as comparing between groups while ANOVA can be used among the three group.P<0.05 prompted that difference was statistically significant.Results:(1)The level of IL-4, TGF-β, IL-17 and the ratio of TGF-β/IL-17 in peripheral blood of CLL patients were higher than those in the control group(P<0.05).The level of IFN-γ and the ratio of IFN-γ/IL-4 were lower than those in the control group(P<0.05). Compared with the CLL group,the treated cases had the lower level of IL-4, TGF-β, IL-17 and TGF-β/IL-17.The ratio of IFN-γ and IFN-γ/IL-4 in treated cases was higher than that in the CLL group.(2) In stage Binet A、B、C,the later the stage,the higher the ratio of TGF-β/IL-17,and the ratio of TGF-β/IL-17 in stage Binet C was higher than that in stage Binet A and Binet B. The later the stage,the lower the ratio of IFN-γ/IL-4. The ratio of IFN-γ/IL-4 and TGF-β/IL-17 were positively correlated with the clinical Binet stages.Conclusions:(1)In our study, the level of cytokines was found that the ratio of IFN-γ/IL-4 and TGF-β/IL-17 change exist in CLL patient. With the progression of the disease,the ratio of IFN-γ/IL-4 decreased gradually,the ratio of TGF-β/IL-17 increased gradually,they could be used as one of the indicators of prognosis.(2) Treatment may contribute to reverse the imbalance of IFN-γ/IL-4 and TGF-β/IL-17.(3) Detection the balance of IFN-γ/IL-4 and TGF-β/IL-17 can indirectly analyze the immune status of the body and the development of the disease, it will provide the basis for the CLL immune therapy model.